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Literature summary for 1.14.14.5 extracted from

  • Eichhorn, E.; van der Ploeg, J.R.; Leisinger, T.
    Characterization of a two-component alkanesulfonate monooxygenase from Escherichia coli (1999), J. Biol. Chem., 274, 26639-26646.
    View publication on PubMed
Search for more literature for 1.14.14.5

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.035
-
 decanesulfonic acid
-
 Escherichia coli
0.044
-
 octanesulfonic acid
-
 Escherichia coli
0.095
-
 hexanesulfonic acid
-
 Escherichia coli
0.11
-
 4-phenyl-1-butanesulfonic acid
-
 Escherichia coli
0.114
-
 1,3-dioxo-2-isoindolineethanesulfonic acid
-
 Escherichia coli
0.139
-
 2-(4-pyridyl)ethanesulfonic acid
-
 Escherichia coli
0.189
-
 pentanesulfonic acid
-
 Escherichia coli
0.237
-
 N-phenyltaurine
-
 Escherichia coli
0.617
-
 MOPS
-
 Escherichia coli
0.87
-
 butanesulfonic acid
-
 Escherichia coli
1.11
-
 PIPES
-
 Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41200
-
 4 * 41200, SDS-PAGE Escherichia coli
181000
-
 gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
R-CH2-SO3H + FMNH2 + O2 Escherichia coli
-
 R-CHO + FMN + sulfite + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 EC1250, chromosomal gene cluster ssuEADCB
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Reaction

Reaction Comment Organism Reaction ID
an alkanesulfonate + FMNH2 + O2 = an aldehyde + FMN + sulfite + H2O absolutely dependent on oxygen Escherichia coli
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5
-
-
 Escherichia coli

Storage Stability

Storage Stability Organism
-20°C, 15% glycerol, the activity increases slightly during the first 2 to 3 weeks of storage Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,3-dioxo-2-isoindolineethanesulfonic acid + FMNH2 + O2
-
 Escherichia coli (1,3-dioxo-1,3-dihydro-2H-isoindol-2-yl)acetaldehyd + FMN + sulfite + H2O
-
 ?
2-(4-pyridyl)ethanesulfonic acid + FMNH2 + O2
-
 Escherichia coli pyridin-4-ylacetaldehyde
-
 ?
4-phenyl-1-butanesulfonic acid + FMNH2 + O2
-
 Escherichia coli 4-phenylbutanol + FMN + sulfite + H2O
-
 ?
butanesulfonic acid + FMNH2 + O2
-
 Escherichia coli butanal + FMN + sulfite + H2O
-
 ?
decanesulfonic acid + FMNH2 + O2
-
 Escherichia coli decanal + FMN + sulfite + H2O
-
 ?
hexanesulfonic acid + FMNH2 + O2
-
 Escherichia coli hexanal + FMN + sulfite + H2O
-
 ?
MOPS + FMNH2 + O2
-
 Escherichia coli ?
-
 ?
additional information no substrates are taurine, methanesulfonic acid, benzenesulfonic acid, L-cysteic acid, ethanedisulfonic acid, toluene-4-sulfonic acid, p-sulfobenzoic acid, benzenesulfonic acid, 4-hydroxybenzenesulfonic acid, SsuD is able to desulfonate C-2 to C-10 unsubstituted alkanesulfonates, substituted ethanesulfonic acids and HEPES, the catalytic efficiency increases with increasing chain length up to decanesulfonic acid Escherichia coli ?
-
 ?
N-phenyltaurine + FMNH2 + O2
-
 Escherichia coli anilinoacetaldehyde + FMN + sulfite + H2O
-
 ?
octanesulfonic acid + FMNH2 + O2
-
 Escherichia coli octanal + FMN + sulfite + H2O
-
 ?
pentanesulfonic acid + FMNH2 + O2
-
 Escherichia coli pentaldehyde + FMN + sulfite + H2O
-
 ?
PIPES + FMNH2 + O2
-
 Escherichia coli ?
-
 ?
R-CH2-SO3H + FMNH2 + O2
-
 Escherichia coli R-CHO + FMN + sulfite + H2O
-
 ?

Subunits

Subunits Comment Organism
homotetramer 4 * 41200, SDS-PAGE Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 enzyme assay Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.1
-
 in 10 mM Tris-HCl Escherichia coli

Cofactor

Cofactor Comment Organism Structure
FMNH2
-
 Escherichia coli