KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0001 | - |
tetrahydrobiopterin | pH 7.6, 25°C | Bacillus subtilis | |
0.0004 | - |
tetrahydrofolate | pH 7.6, 25°C | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | O34453 | - |
- |
Bacillus subtilis 168 | O34453 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 L-arginine + 2 tetrahydrobiopterin + 2 O2 | - |
Bacillus subtilis | 2 Nomega-hydroxy-L-arginine + 2 oxidized tetrahydrobiopterin + 2 H2O | - |
? | |
2 L-arginine + 2 tetrahydrobiopterin + 2 O2 | - |
Bacillus subtilis 168 | 2 Nomega-hydroxy-L-arginine + 2 oxidized tetrahydrobiopterin + 2 H2O | - |
? | |
2 L-arginine + 3 tetrahydrobiopterin + 4 O2 | - |
Bacillus subtilis | 2 L-citrulline + 2 nitric oxide + 3 oxidized tetrahydrobiopterin + 4 H2O | overall reaction | ? | |
2 L-arginine + 3 tetrahydrobiopterin + 4 O2 | - |
Bacillus subtilis 168 | 2 L-citrulline + 2 nitric oxide + 3 oxidized tetrahydrobiopterin + 4 H2O | overall reaction | ? | |
2 L-arginine + 3 tetrahydrofolate + 4 O2 | - |
Bacillus subtilis | 2 L-citrulline + 2 nitric oxide + 3 oxidized tetrahydrofolate + 4 H2O | overall reaction | ? | |
2 L-arginine + 3 tetrahydrofolate + 4 O2 | - |
Bacillus subtilis 168 | 2 L-citrulline + 2 nitric oxide + 3 oxidized tetrahydrofolate + 4 H2O | overall reaction | ? | |
2 Nomega-hydroxy-L-arginine + tetrahydrobiopterin + 2 O2 | - |
Bacillus subtilis | 2 L-citrulline + 2 nitric oxide + oxidized tetrahydrobiopterin + 2 H2O | - |
? | |
2 Nomega-hydroxy-L-arginine + tetrahydrobiopterin + 2 O2 | - |
Bacillus subtilis 168 | 2 L-citrulline + 2 nitric oxide + oxidized tetrahydrobiopterin + 2 H2O | - |
? | |
additional information | ferrous bsNOS reacts with O2 to form a transient heme Fe(II)O2 species in the presence of either Arg or the reaction intermediate N-hydroxy-L-arginine. Disappearance of the Fe(II)O2 species is kinetically and quantitatively coupled to formation of a transient heme Fe(III)NO product, which then dissociates to form ferric NOS. NO formation requires a bound tetrahydropteridine, and the kinetic effects of this cofactor are consistent with it donating an electron to the Fe(II)O2 intermediate during the reaction. Dissociation of the heme Fe(III)NO product is much slower than in mammalian NOS | Bacillus subtilis | ? | - |
? | |
additional information | ferrous bsNOS reacts with O2 to form a transient heme Fe(II)O2 species in the presence of either Arg or the reaction intermediate N-hydroxy-L-arginine. Disappearance of the Fe(II)O2 species is kinetically and quantitatively coupled to formation of a transient heme Fe(III)NO product, which then dissociates to form ferric NOS. NO formation requires a bound tetrahydropteridine, and the kinetic effects of this cofactor are consistent with it donating an electron to the Fe(II)O2 intermediate during the reaction. Dissociation of the heme Fe(III)NO product is much slower than in mammalian NOS | Bacillus subtilis 168 | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.183 | - |
tetrahydrobiopterin | pH 7.6, 25°C | Bacillus subtilis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | - |
Bacillus subtilis | |
tetrahydrobiopterin | NO formation requires a bound tetrahydropteridine, and the kinetic effects are consistent with it donating an electron to the Fe(II)O2 intermediate during the reaction | Bacillus subtilis |