Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.14.45 extracted from

  • Bak, S.; Feyereisen, R.
    The involvement of two p450 enzymes, CYP83B1 and CYP83A1, in auxin homeostasis and glucosinolate biosynthesis (2001), Plant Physiol., 127, 108-118.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.14.45

Inhibitors

Inhibitors Comment Organism Structure
tryptamine Ks value 18 microM Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0031
-
 (E)-indol-3-ylacetaldehyde oxime pH 8.1, 29°C Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(E)-indol-3-ylacetaldehyde oxime + [reduced NADPH-hemoprotein reductase] + glutathione + O2 Arabidopsis thaliana
-
 S-[(E)-N-hydroxy(indol-3-yl)acetimidoyl]-L-glutathione + [oxidized NADPH-hemoprotein reductase] + 2 H2O overall reaction ?
(E)-p-hydroxyphenylacetaldehyde oxime + [reduced NADPH-hemoprotein reductase] + glutathione + O2 Arabidopsis thaliana
-
 S-[(Z)-N-hydroxy(p-hydroxyphenyl)acetimidoyl]-L-glutathione + [oxidized NADPH-hemoprotein reductase] + 2 H2O overall reaction ?
(E)-phenylacetaldehyde oxime + [reduced NADPH-hemoprotein reductase] + glutathione + O2 Arabidopsis thaliana
-
 S-[(Z)-N-hydroxy(phenyl)acetimidoyl]-L-glutathione + [oxidized NADPH-hemoprotein reductase] + 2 H2O overall reaction ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana O65782
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(E)-indol-3-ylacetaldehyde oxime + [reduced NADPH-hemoprotein reductase] + glutathione + O2
-
 Arabidopsis thaliana S-[(E)-N-hydroxy(indol-3-yl)acetimidoyl]-L-glutathione + [oxidized NADPH-hemoprotein reductase] + 2 H2O overall reaction ?
(E)-p-hydroxyphenylacetaldehyde oxime + [reduced NADPH-hemoprotein reductase] + glutathione + O2
-
 Arabidopsis thaliana S-[(Z)-N-hydroxy(p-hydroxyphenyl)acetimidoyl]-L-glutathione + [oxidized NADPH-hemoprotein reductase] + 2 H2O overall reaction ?
(E)-phenylacetaldehyde oxime + [reduced NADPH-hemoprotein reductase] + glutathione + O2
-
 Arabidopsis thaliana S-[(Z)-N-hydroxy(phenyl)acetimidoyl]-L-glutathione + [oxidized NADPH-hemoprotein reductase] + 2 H2O overall reaction ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.16
-
 (E)-p-hydroxyphenylacetaldehyde oxime pH 8.1, 29°C Arabidopsis thaliana
0.25
-
 (E)-phenylacetaldehyde oxime pH 8.1, 29°C Arabidopsis thaliana
0.43
-
 (E)-indol-3-ylacetaldehyde oxime pH 8.1, 29°C Arabidopsis thaliana

General Information

General Information Comment Organism
physiological function lack of the CYP83B1 gene product leads to auxin excess and indole glucosinolate deficit, ectopic overexpression of CYP83A1 using a 35S promoter rescues phenotype. Indole-3-acetaldoxime has a 50-fold higher affinity toward CYP83B1 than toward CYP83A1, EC 1.14.14.43. Both enzymes also metabolize the phenylalanine- and tyrosine-derived aldoximes. Indole-3-acetaldoxime is the physiological substrate for CYP83B1 Arabidopsis thaliana