Literature summary for 1.14.14.45 extracted from

Hansen, C.H.; Du, L.; Naur, P.; Olsen, C.E.; Axelsen, K.B.; Hick, A.J.; Pickett, J.A.; Halkier, B.A.
CYP83B1 is the oxime-metabolizing enzyme in the glucosinolate pathway in Arabidopsis (2001), J. Biol. Chem., 276, 24790-24796.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Arabidopsis thaliana

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.068	-	p-hydroxyphenylacetaldoxime	pH 81, 29°C	Arabidopsis thaliana

Organism

Organism	UniProt	Comment	Textmining
Arabidopsis thaliana	O65782	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
p-hydroxyphenylacetaldoxime + [reduced NADPH-hemoprotein reductase] + cysteine + O2	-	Arabidopsis thaliana	S-(benzohydroximoyl)-L-cysteine + [oxidized NADPH-hemoprotein reductase] + 2 H2O	overall reaction	?
p-hydroxyphenylacetaldoxime + [reduced NADPH-hemoprotein reductase] + N-acetylcysteine + O2	-	Arabidopsis thaliana	S-(benzohydroximoyl)-N-acetyl-L-cysteine + [oxidized NADPH-hemoprotein reductase] + 2 H2O	overall reaction	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.27	-	p-hydroxyphenylacetaldoxime	pH 8.1, 29°C	Arabidopsis thaliana

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8.1	-	-	Arabidopsis thaliana

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Release 2023.1 (January 2023)