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Literature summary for 1.14.14.44 extracted from

  • Yamaguchi, T.; Yamamoto, K.; Asano, Y.
    Identification and characterization of CYP79D16 and CYP71AN24 catalyzing the first and second steps in L-phenylalanine-derived cyanogenic glycoside biosynthesis in the Japanese apricot, Prunus mume Sieb. et Zucc (2014), Plant Mol. Biol., 86, 215-223.
    View publication on PubMed
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KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0039
-
 (E)-phenylacetaldehyde oxime pH 7.6, 30°C Prunus mume
0.007
-
 4-hydroxyphenylacetaldoxime pH 7.6, 30°C Prunus mume
0.0082
-
 indole-3-acetaldoxime pH 7.6, 30°C Prunus mume
0.0185
-
 phenylacetonitrile pH 7.6, 30°C Prunus mume
0.195
-
 4-Hydroxyphenylacetonitrile pH 7.6, 30°C Prunus mume
0.751
-
 indole-3-acetonitrile pH 7.6, 30°C Prunus mume

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(E)-phenylacetaldehyde oxime Prunus mume
-
 (Z)-phenylacetaldehyde oxime
-
 ?
(E)-phenylacetaldehyde oxime + [reduced NADPH-hemoprotein reductase] + O2 Prunus mume
-
 (R)-mandelonitrile + [oxidized NADPH-hemoprotein reductase] + 2 H2O overall reaction ?
(Z)-phenylacetaldehyde oxime Prunus mume
-
 phenylacetonitrile + H2O
-
 ?
phenylacetonitrile + [reduced NADPH-hemoprotein reductase] + O2 Prunus mume
-
 (R)-mandelonitrile + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Prunus mume A0A068Q609 var. Sieb. et Zucc.
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Prunus mume
-
seedling
-
 Prunus mume
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(E)-phenylacetaldehyde oxime
-
 Prunus mume (Z)-phenylacetaldehyde oxime
-
 ?
(E)-phenylacetaldehyde oxime + [reduced NADPH-hemoprotein reductase] + O2
-
 Prunus mume (R)-mandelonitrile + [oxidized NADPH-hemoprotein reductase] + 2 H2O overall reaction ?
(Z)-phenylacetaldehyde oxime
-
 Prunus mume phenylacetonitrile + H2O
-
 ?
4-hydroxyphenylacetaldoxime + [reduced NADPH-hemoprotein reductase] + O2
-
 Prunus mume hydroxy(4-hydroxyphenyl)acetonitrile + [oxidized NADPH-hemoprotein reductase] + 2 H2O overall reaction ?
4-hydroxyphenylacetonitrile + [reduced NADPH-hemoprotein reductase] + O2
-
 Prunus mume hydroxy(4-hydroxyphenyl)acetonitrile + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
 ?
indole-3-acetaldoxime + [reduced NADPH-hemoprotein reductase] + O2 reaction of EC 1.14.14.45 Prunus mume hydroxy(1H-indol-3-yl)acetonitrile + [oxidized NADPH-hemoprotein reductase] + 2 H2O overall reaction ?
indole-3-acetonitrile + [reduced NADPH-hemoprotein reductase] + O2 reaction of EC 1.14.14.45 Prunus mume hydroxy(1H-indol-3-yl)acetonitrile + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
 ?
additional information enzyme converts aromatic aldoximes and nitriles to the corresponding hydroxynitriles. No substrates: aliphatic substrates 2-methylpropanal oxime and 2-methylpropanenitrile Prunus mume ?
-
 ?
phenylacetonitrile + [reduced NADPH-hemoprotein reductase] + O2
-
 Prunus mume (R)-mandelonitrile + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Synonyms

Synonyms Comment Organism
CYP71AN24
-
 Prunus mume

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.028
-
 indole-3-acetaldoxime pH 7.6, 30°C Prunus mume
0.1
-
 indole-3-acetonitrile pH 7.6, 30°C Prunus mume
0.4
-
 phenylacetonitrile pH 7.6, 30°C Prunus mume
0.57
-
 4-Hydroxyphenylacetonitrile pH 7.6, 30°C Prunus mume
0.62
-
 4-hydroxyphenylacetaldoxime pH 7.6, 30°C Prunus mume
0.77
-
 (E)-phenylacetaldehyde oxime pH 7.6, 30°C Prunus mume

Cofactor

Cofactor Comment Organism Structure
cytochrome P450
-
 Prunus mume