Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Arabidopsis thaliana |
expression in Saccharomyces cerevisiae | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.026 | - |
L-hexahomomethionine | pH 7.5, 25°C | Arabidopsis thaliana | |
0.034 | - |
L-dihomomethionine | pH 7.5, 25°C | Arabidopsis thaliana | |
0.037 | - |
L-trihomomethionine | pH 7.5, 25°C | Arabidopsis thaliana | |
0.074 | - |
L-hexahomomethionine | pH 7.5, 25°C | Arabidopsis thaliana | |
0.194 | - |
L-tetrahomomethionine | pH 7.5, 25°C | Arabidopsis thaliana | |
0.216 | - |
L-pentahomomethionine | pH 7.5, 25°C | Arabidopsis thaliana | |
0.374 | - |
L-pentahomomethionine | pH 7.5, 25°C | Arabidopsis thaliana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
62000 | - |
- |
Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-dihomomethionine + 2 O2 + 2 [reduced NADPH-hemoprotein reductase] | Arabidopsis thaliana | - |
4-methylthiopentanaldoxime + CO2 + 3 H2O + 2 [oxidized NADPH-hemoprotein reductase] | - |
? | |
L-hexahomomethionine + 2 O2 + 2 [reduced NADPH-hemoprotein reductase] | Arabidopsis thaliana | - |
4-methylthiononanaldoxime + CO2 + 3 H2O + 2 [oxidized NADPH-hemoprotein reductase] | overall reaction | ? | |
L-homomethionine + 2 O2 + 2 [reduced NADPH-hemoprotein reductase] | Arabidopsis thaliana | - |
4-methylthiobutanaldoxime + CO2 + 3 H2O + 2 [oxidized NADPH-hemoprotein reductase] | - |
? | |
L-pentahomomethionine + 2 O2 + 2 [reduced NADPH-hemoprotein reductase] | Arabidopsis thaliana | - |
4-methylthiooctanaldoxime + CO2 + 3 H2O + 2 [oxidized NADPH-hemoprotein reductase] | overall reaction | ? | |
L-tetrahomomethionine + 2 O2 + 2 [reduced NADPH-hemoprotein reductase] | Arabidopsis thaliana | - |
4-methylthioheptanaldoxime + CO2 + 3 H2O + 2 [oxidized NADPH-hemoprotein reductase] | overall reaction | ? | |
L-trihomomethionine + 2 O2 + 2 [reduced NADPH-hemoprotein reductase] | Arabidopsis thaliana | - |
4-methylthiohexanaldoxime + CO2 + 3 H2O + 2 [oxidized NADPH-hemoprotein reductase] | overall reaction | ? | |
additional information | Arabidopsis thaliana | isoform CYP79F1 metabolizes mono- to hexahomomethionine, resulting in both short- and long-chain aliphatic glucosinolates. No substrates: phenylalanine,homophenylalanine, tyrosine, tryptophan, and methionine | ? | - |
? | |
additional information | Arabidopsis thaliana | isoform CYP79F2 exclusively metabolizes long-chain elongated penta- and hexahomomethionines. No substrates: phenylalanine, homophenylalanine, tyrosine, tryptophan, and methionine | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q949U1 | - |
- |
Arabidopsis thaliana | Q9FUY7 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cotyledon | strongly expressed in cotyledons, rosette leaves, stems, and siliques | Arabidopsis thaliana | - |
hypocotyl | strongly expressed in hypocotyl and root | Arabidopsis thaliana | - |
leaf | strongly expressed in cotyledons, rosette leaves, stems, and siliques | Arabidopsis thaliana | - |
root | strongly expressed in hypocotyl and root | Arabidopsis thaliana | - |
silique | strongly expressed in cotyledons, rosette leaves, stems, and siliques | Arabidopsis thaliana | - |
stem | strongly expressed in cotyledons, rosette leaves, stems, and siliques | Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-dihomomethionine + 2 O2 + 2 [reduced NADPH-hemoprotein reductase] | - |
Arabidopsis thaliana | 4-methylthiopentanaldoxime + CO2 + 3 H2O + 2 [oxidized NADPH-hemoprotein reductase] | - |
? | |
L-hexahomomethionine + 2 O2 + 2 [reduced NADPH-hemoprotein reductase] | - |
Arabidopsis thaliana | 4-methylthiononanaldoxime + CO2 + 3 H2O + 2 [oxidized NADPH-hemoprotein reductase] | overall reaction | ? | |
L-homomethionine + 2 O2 + 2 [reduced NADPH-hemoprotein reductase] | - |
Arabidopsis thaliana | 4-methylthiobutanaldoxime + CO2 + 3 H2O + 2 [oxidized NADPH-hemoprotein reductase] | - |
? | |
L-pentahomomethionine + 2 O2 + 2 [reduced NADPH-hemoprotein reductase] | - |
Arabidopsis thaliana | 4-methylthiooctanaldoxime + CO2 + 3 H2O + 2 [oxidized NADPH-hemoprotein reductase] | overall reaction | ? | |
L-tetrahomomethionine + 2 O2 + 2 [reduced NADPH-hemoprotein reductase] | - |
Arabidopsis thaliana | 4-methylthioheptanaldoxime + CO2 + 3 H2O + 2 [oxidized NADPH-hemoprotein reductase] | overall reaction | ? | |
L-trihomomethionine + 2 O2 + 2 [reduced NADPH-hemoprotein reductase] | - |
Arabidopsis thaliana | 4-methylthiohexanaldoxime + CO2 + 3 H2O + 2 [oxidized NADPH-hemoprotein reductase] | overall reaction | ? | |
additional information | isoform CYP79F1 metabolizes mono- to hexahomomethionine, resulting in both short- and long-chain aliphatic glucosinolates. No substrates: phenylalanine,homophenylalanine, tyrosine, tryptophan, and methionine | Arabidopsis thaliana | ? | - |
? | |
additional information | isoform CYP79F2 exclusively metabolizes long-chain elongated penta- and hexahomomethionines. No substrates: phenylalanine, homophenylalanine, tyrosine, tryptophan, and methionine | Arabidopsis thaliana | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 62000, calculated and SDS-PAGE | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
CYP79F1 | - |
Arabidopsis thaliana |
CYP79F2 | - |
Arabidopsis thaliana |
hexahomomethionine N-hydroxylase | - |
Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
physiological function | a transposon-tagged CYP79F1 knockout mutant completely lacks short-chain aliphatic glucosinolates, but has an increased level of long-chain aliphatic glucosinolates, especially in leaves and seeds | Arabidopsis thaliana |
physiological function | the level of long-chain aliphatic glucosinolates in a transposon-tagged CYP79F2 knockout mutant is substantially reduced, whereas the level of short-chain aliphatic glucosinolates is not affected | Arabidopsis thaliana |