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Literature summary for 1.14.14.41 extracted from

  • Jorgensen, K.; Morant, A.V.; Morant, M.; Jensen, N.B.; Olsen, C.E.; Kannangara, R.; Motawia, M.S.; Moller, B.L.; Bak, S.
    Biosynthesis of the cyanogenic glucosides linamarin and lotaustralin in cassava: isolation, biochemical characterization, and expression pattern of CYP71E7, the oxime-metabolizing cytochrome P450 enzyme (2011), Plant Physiol., 155, 282-292.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.14.41

Inhibitors

Inhibitors Comment Organism Structure
n-octylamine competitive Manihot esculenta

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.021
-
 (E)-2-methylbutanal oxime pH 7.9, 28°C Manihot esculenta

Organism

Organism UniProt Comment Textmining
Manihot esculenta Q6XQ14
-
-

Source Tissue

Source Tissue Comment Organism Textmining
endodermis of nearly unfolded leaf Manihot esculenta
-
leaf in nearly unfolded leaves, the CYP71E7 paralogs are preferentially expressed in specific cells in the endodermis and in most cells in the first cortex cell layer. In fully unfolded leaves, the expression is pronounced in the cortex cell layer just beside the epidermis and in specific cells in the vascular tissue cortex cells Manihot esculenta
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(E)-2-methylbutanal oxime
-
 Manihot esculenta (Z)-2-methylbutanal oxime
-
 ?
(E)-2-methylbutanal oxime + [reduced NADPH-hemoprotein reductase] + O2 i.e. isoleucine-derived oxime Manihot esculenta 2-hydroxy-2-methylbutanenitrile + [oxidized NADPH-hemoprotein reductase] + 2 H2O overall reaction ?
(E)-2-methylpropanal oxime
-
 Manihot esculenta (Z)-2-methylpropanal oxime
-
 ?
(E)-2-methylpropanal oxime + [reduced NADPH-hemoprotein reductase] + O2 i.e. valine-derived oxime Manihot esculenta 2-hydroxy-2-methylpropanenitrile + [oxidized NADPH-hemoprotein reductase] + 2 H2O overall reaction ?
(Z)-2-methylbutanal oxime
-
 Manihot esculenta 2-methylbutanenitrile + H2O
-
 ?
(Z)-2-methylpropanal oxime
-
 Manihot esculenta 2-methylpropanenitrile + H2O
-
 ?
2-methylbutanenitrile + [reduced NADPH-hemoprotein reductase] + O2
-
 Manihot esculenta 2-hydroxy-2-methylbutanenitrile + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
2-methylpropanenitrile + [reduced NADPH-hemoprotein reductase] + O2
-
 Manihot esculenta 2-hydroxy-2-methylpropanenitrile + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
additional information CYP71E7 exhibits low specificity for the side chain of the substrate and catalyzes the conversion of aliphatic and aromatic oximes Manihot esculenta ?
-
 ?
N-hydroxy-2-(4-hydroxypheny)-lethanimine + [reduced NADPH-hemoprotein reductase] + O2 i.e. phenylalanine-derived oxime Manihot esculenta (4-hydroxyphenyl)acetonitrile + [oxidized NADPH-hemoprotein reductase] + 2 H2O overall reaction ?
N-hydroxy-2-phenylethanimine + [reduced NADPH-hemoprotein reductase] + O2 i.e. phenylalanine-derived oxime Manihot esculenta phenylacetonitrile + [oxidized NADPH-hemoprotein reductase] + 2 H2O overall reaction ?

Synonyms

Synonyms Comment Organism
CYP71E7
-
 Manihot esculenta

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.02
-
 N-hydroxy-2-phenylethanimine pH 7.9, 28°C Manihot esculenta
0.13
-
 N-hydroxy-2-(4-hydroxypheny)-lethanimine pH 7.9, 28°C Manihot esculenta
0.28
-
 (E)-2-methylbutanal oxime pH 7.9, 28°C Manihot esculenta
0.35
-
 (E)-2-methylpropanal oxime pH 7.9, 28°C Manihot esculenta

Cofactor

Cofactor Comment Organism Structure
heme
-
 Manihot esculenta

General Information

General Information Comment Organism
physiological function CYP71E7 is the oxime-metabolizing cytochrome P450 in the biosynthesis of lotaustralin and linamarin. Expression of CYP71E7 in yeast affords microsomes converting 2-methylpropanal oxime and 2-methylbutanal oxime to the corresponding cyanohydrins, which dissociate into acetone and 2-butanone, respectively, and hydrogen cyanide Manihot esculenta