Literature summary for 1.14.14.40 extracted from

Miki, Y.; Asano, Y.
Construction of the biosynthetic pathway for the cyanide-free production of phenylacetonitrile in Escherichia coli utilizing plant cytochrome P450 79A2 and bacterial aldoxime dehydratase (2014), Appl. Environ. Microbiol., 80, 6828-6836.

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Application

Application	Comment	Organism
synthesis	biosynthetic pathway for the production of phenylacetonitrile in Escherichia coli utilizing enzymes from the plant glucosinolate-biosynthetic and bacterial aldoxime-nitrile pathways, i.e. introducing the genes encoding cytochrome P450 (CYP) 79A2 and CYP reductase from Arabidopsis thaliana, yielding the E,Z-phenylacetaldoxime-producing transformant and further introducing the aldoxime dehydratase (Oxd) gene from Bacillus sp. strain OxB-1, yielding the phenylacetonitrile-producing transformant. Expression of active CYP79A2 and concentration of biomass are improved by the combination of the autoinduction method, coexpression of groE, encoding the heat shock protein GroEL/GroES, N-terminal truncation of CYP79A2, and optimization of the culture conditions, yielding a more than 60fold concentration of E,Z-phenylacetaldoxime up to 2.9 mM	Arabidopsis thaliana

Organism

Organism	UniProt	Comment	Textmining
Arabidopsis thaliana	Q9FLC8	-	-

Synonyms

Synonyms	Comment	Organism
CYP79A2	-	Arabidopsis thaliana

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Release 2023.1 (January 2023)