Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Sorghum bicolor | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(E)-4-hydroxyphenylacetaldehyde oxime + [reduced NADPH-hemoprotein reductase] + O2 | Sorghum bicolor | involved in dhurrin synthesis | (S)-4-hydroxymandelonitrile + [oxidized NADPH-hemoprotein reductase] + 2 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sorghum bicolor | - |
L. Moench | - |
Sorghum bicolor | O48958 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | the site of dhurrin synthesis shifts from leaves to stem during plant development. At all stages, the content of dhurrin correlates well with the activity of the two biosynthetic enzymes, CYP79A1 and CYP71E1, and with the protein and mRNA level for the two enzymes | Sorghum bicolor | - |
seed | maximal activity after 1 day of germination | Sorghum bicolor | - |
stem | the site of dhurrin synthesis shifts from leaves to stem during plant development. At all stages, the content of dhurrin correlates well with the activity of the two biosynthetic enzymes, CYP79A1 and CYP71E1, and with the protein and mRNA level for the two enzymes | Sorghum bicolor | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(E)-4-hydroxyphenylacetaldehyde oxime + [reduced NADPH-hemoprotein reductase] + O2 | - |
Sorghum bicolor | (S)-4-hydroxymandelonitrile + [oxidized NADPH-hemoprotein reductase] + 2 H2O | - |
? | |
(E)-4-hydroxyphenylacetaldehyde oxime + [reduced NADPH-hemoprotein reductase] + O2 | involved in dhurrin synthesis | Sorghum bicolor | (S)-4-hydroxymandelonitrile + [oxidized NADPH-hemoprotein reductase] + 2 H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CYP71E1 | - |
Sorghum bicolor |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Sorghum bicolor |
General Information | Comment | Organism |
---|---|---|
physiological function | at all stages, the content of dhurrin correlates well with the activity of the two biosynthetic enzymes, CYP79A1 and CYP71E1, and with the protein and mRNA level for the two enzymes. During development, the activity of tyrosine N-monooxygenase CYP79A1 is lower than the activity of 4-hydroxyphenylacetaldehyde oxime monooxygenase CYP71E1, suggesting that CYP79A1 catalyzes the rate-limiting step in dhurrin synthesis | Sorghum bicolor |