Application | Comment | Organism |
---|---|---|
biotechnology | engineering of the dhurrin pathway from Sorghum bicolor into the chloroplasts of Nicotiana tabacum. The entire pathway can be introduced into the chloroplast by integrating membrane-bound cytochrome P450 enzymes CYP79A1, CYP71E1, and soluble glucosyltransferase UGT85B1 into a neutral site of the Nicotiana tabacum chloroplast genome. The two P450s and the UGT85B1 are functional when expressed in the chloroplasts and convert endogenous tyrosine into dhurrin using electrons derived directly from the photosynthetic electron transport chain, without the need for the presence of an NADPH-dependent P450 oxidoreductase. The dhurrin produced in the engineered plants amounts to 0.10.2% of leaf dry weight compared to 6% in sorghum | Sorghum bicolor |
Organism | UniProt | Comment | Textmining |
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Sorghum bicolor | Q43135 | - |
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