BRENDA - Enzyme Database show
show all sequences of 1.14.14.34

A novel reduced flavin mononucleotide-dependent methanesulfonate sulfonatase encoded by the sulfur-regulated msu operon of Pseudomonas aeruginosa

Kertesz, M.A.; Schmidt-Larbig, K.; Wueest, T.; J. Bacteriol. 181, 1464-1473 (1999)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli
Pseudomonas aeruginosa
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
41600
-
-
Pseudomonas aeruginosa
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas aeruginosa
Q9I1C2
-
-
Pseudomonas aeruginosa DSM 22644
Q9I1C2
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ethanesulfonate + FMNH2 + O2
27% of the activity with methanesulfonate
738509
Pseudomonas aeruginosa
acetaldehyde + FMN + sulfite + H2O
-
-
-
?
ethanesulfonate + FMNH2 + O2
27% of the activity with methanesulfonate
738509
Pseudomonas aeruginosa DSM 22644
acetaldehyde + FMN + sulfite + H2O
-
-
-
?
isethionate + FMNH2 + O2
19% of the activity with methanesulfonate
738509
Pseudomonas aeruginosa
hydroxyacetaldehyde + FMN + sulfite + H2O
-
-
-
?
isethionate + FMNH2 + O2
19% of the activity with methanesulfonate
738509
Pseudomonas aeruginosa DSM 22644
hydroxyacetaldehyde + FMN + sulfite + H2O
-
-
-
?
methanesulfonate + FMNH2 + O2
-
738509
Pseudomonas aeruginosa
formaldehyde + FMN + sulfite + H2O
-
-
-
?
methanesulfonate + FMNH2 + O2
-
738509
Pseudomonas aeruginosa DSM 22644
formaldehyde + FMN + sulfite + H2O
-
-
-
?
morpholinepropanesulfonic acid + FMNH2 + O2
17% of the activity with methanesulfonate
738509
Pseudomonas aeruginosa
3-(morpholin-4-yl)propanal + FMN + sulfite + H2O
-
-
-
?
morpholinepropanesulfonic acid + FMNH2 + O2
17% of the activity with methanesulfonate
738509
Pseudomonas aeruginosa DSM 22644
3-(morpholin-4-yl)propanal + FMN + sulfite + H2O
-
-
-
?
pentanesulfonate + FMNH2 + O2
13% of the activity with methanesulfonate
738509
Pseudomonas aeruginosa
pentanal + FMN + sulfite + H2O
-
-
-
?
pentanesulfonate + FMNH2 + O2
13% of the activity with methanesulfonate
738509
Pseudomonas aeruginosa DSM 22644
pentanal + FMN + sulfite + H2O
-
-
-
?
piperazine-N,N'-bis(2-ethanesulfonic acid) + 2 FMNH2 + 2 O2
31% of the activity with methanesulfonate
738509
Pseudomonas aeruginosa
2,2'-piperazine-1,4-diyldiacetaldehyde + 2 FMN + 2 sulfite + 2 H2O
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 41600, calculated
Pseudomonas aeruginosa
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Pseudomonas aeruginosa
calculated
-
6.6
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Pseudomonas aeruginosa
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
41600
-
-
Pseudomonas aeruginosa
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ethanesulfonate + FMNH2 + O2
27% of the activity with methanesulfonate
738509
Pseudomonas aeruginosa
acetaldehyde + FMN + sulfite + H2O
-
-
-
?
ethanesulfonate + FMNH2 + O2
27% of the activity with methanesulfonate
738509
Pseudomonas aeruginosa DSM 22644
acetaldehyde + FMN + sulfite + H2O
-
-
-
?
isethionate + FMNH2 + O2
19% of the activity with methanesulfonate
738509
Pseudomonas aeruginosa
hydroxyacetaldehyde + FMN + sulfite + H2O
-
-
-
?
isethionate + FMNH2 + O2
19% of the activity with methanesulfonate
738509
Pseudomonas aeruginosa DSM 22644
hydroxyacetaldehyde + FMN + sulfite + H2O
-
-
-
?
methanesulfonate + FMNH2 + O2
-
738509
Pseudomonas aeruginosa
formaldehyde + FMN + sulfite + H2O
-
-
-
?
methanesulfonate + FMNH2 + O2
-
738509
Pseudomonas aeruginosa DSM 22644
formaldehyde + FMN + sulfite + H2O
-
-
-
?
morpholinepropanesulfonic acid + FMNH2 + O2
17% of the activity with methanesulfonate
738509
Pseudomonas aeruginosa
3-(morpholin-4-yl)propanal + FMN + sulfite + H2O
-
-
-
?
morpholinepropanesulfonic acid + FMNH2 + O2
17% of the activity with methanesulfonate
738509
Pseudomonas aeruginosa DSM 22644
3-(morpholin-4-yl)propanal + FMN + sulfite + H2O
-
-
-
?
pentanesulfonate + FMNH2 + O2
13% of the activity with methanesulfonate
738509
Pseudomonas aeruginosa
pentanal + FMN + sulfite + H2O
-
-
-
?
pentanesulfonate + FMNH2 + O2
13% of the activity with methanesulfonate
738509
Pseudomonas aeruginosa DSM 22644
pentanal + FMN + sulfite + H2O
-
-
-
?
piperazine-N,N'-bis(2-ethanesulfonic acid) + 2 FMNH2 + 2 O2
31% of the activity with methanesulfonate
738509
Pseudomonas aeruginosa
2,2'-piperazine-1,4-diyldiacetaldehyde + 2 FMN + 2 sulfite + 2 H2O
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 41600, calculated
Pseudomonas aeruginosa
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Pseudomonas aeruginosa
calculated
-
6.6
Expression
Organism
Commentary
Expression
Pseudomonas aeruginosa
expression of the msu operon is repressed in the presence of sulfate, sulfite, sulfide, or cysteine
down
Pseudomonas aeruginosa
growth with methanesulfonate requires an intact cysB gene, and the msu operon is part of the cys regulon
additional information
Pseudomonas aeruginosa
expression of the msu operon is derepressed during growth with methionine or alkanesulfonates
up
General Information
General Information
Commentary
Organism
physiological function
the MsuD protein catalyzes the desulfonation of alkanesulfonates, requiring oxygen and FMNH2 for the reaction, and shows highest activity with methanesulfonate. NADH-dependent flavin mononucleotide reductase MsuE provides reduced FMN for the MsuD enzyme. Putative monooxygenase MsuC is involved but not essential in the desulfonation process
Pseudomonas aeruginosa
General Information (protein specific)
General Information
Commentary
Organism
physiological function
the MsuD protein catalyzes the desulfonation of alkanesulfonates, requiring oxygen and FMNH2 for the reaction, and shows highest activity with methanesulfonate. NADH-dependent flavin mononucleotide reductase MsuE provides reduced FMN for the MsuD enzyme. Putative monooxygenase MsuC is involved but not essential in the desulfonation process
Pseudomonas aeruginosa
Expression (protein specific)
Organism
Commentary
Expression
Pseudomonas aeruginosa
expression of the msu operon is repressed in the presence of sulfate, sulfite, sulfide, or cysteine
down
Pseudomonas aeruginosa
growth with methanesulfonate requires an intact cysB gene, and the msu operon is part of the cys regulon
additional information
Pseudomonas aeruginosa
expression of the msu operon is derepressed during growth with methionine or alkanesulfonates
up
Other publictions for EC 1.14.14.34
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
737523
Endoh
Characterization and identific ...
Pseudomonas putida, Pseudomonas putida DS1
Appl. Microbiol. Biotechnol.
62
83-91
2003
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6
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2
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2
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6
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1
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1
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6
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2
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6
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1
1
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713986
Borodina
Dimethylsulfone as a growth su ...
Hyphomicrobium sulfonivorans
Arch. Microbiol.
173
425-437
2000
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1
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2
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2
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738509
Kertesz
A novel reduced flavin mononuc ...
Pseudomonas aeruginosa, Pseudomonas aeruginosa DSM 22644
J. Bacteriol.
181
1464-1473
1999
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-
1
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1
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2
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11
1
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1
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1
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1
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11
1
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1
3
1
1
3
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