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Literature summary for 1.14.14.3 extracted from
- The fusion Vibrio campbellii luciferase as a eukaryotic gene reporter (2012), J. Biotechnol., 162, 346-353.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) and HEK-293T cells | Vibrio campbellii |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 77000 | - |
2 * 77000, SDS-PAGE | Vibrio campbellii |
| 77800 | - |
2 * 77800, calculated from amino acid sequence | Vibrio campbellii |
| 158000 | - |
gel filtration | Vibrio campbellii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Vibrio campbellii | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| RCHO + FMNH2 + O2 | - |
Vibrio campbellii | RCOOH + FMN + H2O + hv | the enzyme is emitting blue-green light at 490 nm | ? |  |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 77000, SDS-PAGE | Vibrio campbellii |
| homodimer | 2 * 77800, calculated from amino acid sequence | Vibrio campbellii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| luciferase | - |
Vibrio campbellii |
| Lux | - |
Vibrio campbellii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 37 | half-life of about 1020 min at 37°C. At 37°C, the native enzyme retains around 60% of the bioluminescence of the enzyme expressed at 30°C | Vibrio campbellii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Vibrio campbellii |
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Release 2023.1 (January 2023)
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