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Literature summary for 1.14.14.3 extracted from

  • Wiles, S.; Ferguson, K.; Stefanidou, M.; Young, D.B.; Robertson, B.D.
    Alternative luciferase for monitoring bacterial cells under adverse conditions (2005), Appl. Environ. Microbiol., 71, 3427-3432.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene Gluc, expression in Mycobacterium smegmatis using an hsp60 promoter, subcloning in Escherichia coli strain DH5alpha Gaussia princeps

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
coelenterazine + FMNH2 + O2 Gaussia princeps an imidazolopyrazine derivative CO2 + coelenteramide + FMN + light + H2O
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ir
additional information Gaussia princeps the Gluc luciferase retains its luminescence output in the stationary phase of growth and exhibits enhanced stability during exposure to low pH, hydrogen peroxide, and high temperature ?
-
?

Organism

Organism UniProt Comment Textmining
Gaussia princeps
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gene Gluc
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Oxidation Stability

Oxidation Stability Organism
the enzyme exhibits enhanced stability during exposure to low pH, hydrogen peroxide, and high temperature Gaussia princeps

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
coelenterazine + FMNH2 + O2 an imidazolopyrazine derivative Gaussia princeps CO2 + coelenteramide + FMN + light + H2O
-
ir
additional information the Gluc luciferase retains its luminescence output in the stationary phase of growth and exhibits enhanced stability during exposure to low pH, hydrogen peroxide, and high temperature Gaussia princeps ?
-
?

Synonyms

Synonyms Comment Organism
Gluc luciferase
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Gaussia princeps
luciferase
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Gaussia princeps

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
the enzyme exhibits enhanced stability during exposure to low pH, hydrogen peroxide, and high temperature Gaussia princeps

pH Stability

pH Stability pH Stability Maximum Comment Organism
additional information
-
the enzyme exhibits enhanced stability during exposure to low pH, hydrogen peroxide, and high temperature Gaussia princeps

Cofactor

Cofactor Comment Organism Structure
FMNH2
-
Gaussia princeps