Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes in Escherichia coli | Vibrio harveyi |
Protein Variants | Comment | Organism |
---|---|---|
A81H | site-directed mutagenesis, residue of the alpha-subunit, mutant shows 13% of wild-type activity | Vibrio harveyi |
H81A | site-directed mutagenesis, residue of the beta-subunit, mutant shows 59% of wild-type activity | Vibrio harveyi |
H81A/E89D | site-directed mutagenesis, residues of the beta-subunit, mutant shows 13% of wild-type activity | Vibrio harveyi |
H82A | site-directed mutagenesis, residue of the beta-subunit, mutant shows 22% of wild-type activity | Vibrio harveyi |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Urea | denaturation curve, thermodynamics, wild-type and mutants, overview | Vibrio harveyi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | detailed reaction and folding kinetics, thermodynamics | Vibrio harveyi |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
75430 | - |
wild-type enzyme, analytical ultracentrifugation | Vibrio harveyi |
76360 | - |
mutant beta-H82A, analytical ultracentrifugation | Vibrio harveyi |
76740 | - |
mutant beta-H81A, analytical ultracentrifugation | Vibrio harveyi |
76750 | - |
mutant beta-H81A/E89D, analytical ultracentrifugation | Vibrio harveyi |
76860 | - |
mutant alpha-A81H, analytical ultracentrifugation | Vibrio harveyi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vibrio harveyi | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
RCHO + FMNH2 + O2 | - |
Vibrio harveyi | RCOOH + FMN + H2O + hn | - |
ir |
Subunits | Comment | Organism |
---|---|---|
More | analysis of subunit interface structure and role in the conformational stability of the heterodimeric enzyme, the beta-sunbunit can self-associate to form a stable but inactive homodimer, unfolding in a four-state mechanism | Vibrio harveyi |
Synonyms | Comment | Organism |
---|---|---|
bacterial luciferase | - |
Vibrio harveyi |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Vibrio harveyi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Vibrio harveyi |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMNH2 | - |
Vibrio harveyi |