Cloned (Comment) | Organism |
---|---|
gene luxA, expression of wild-type and mutant enzymes in Escherichia coli strain JM109 | Vibrio harveyi |
Protein Variants | Comment | Organism |
---|---|---|
F261A | site-directed mutagenesis, residue of the alpha-subunit, 0.19% of the wild-type activity, the bulky and hydrophobic nature of the alphaF261 residue is critical for activity | Vibrio harveyi |
F261D | site-directed mutagenesis, residue of the alpha-subunit, 0.004% of the wild-type activity, the bulky and hydrophobic nature of the alphaF261 residue is critical for activity | Vibrio harveyi |
F261S | site-directed mutagenesis, residue of the alpha-subunit, 0.13% of the wild-type activity, the bulky and hydrophobic nature of the alphaF261 residue is critical for activity | Vibrio harveyi |
F261Y | site-directed mutagenesis, residue of the alpha-subunit, 2-3% of the wild-type activity, the bulky and hydrophobic nature of the alphaF261 residue is critical for activity | Vibrio harveyi |
G275A | site-directed mutagenesis, residue of the alpha-subunit, 27% of the wild-type activity, the torsional flexibility of the alphaG275 residue is critical for activity | Vibrio harveyi |
G275F | site-directed mutagenesis, residue of the alpha-subunit, 6-7% of the wild-type activity, the torsional flexibility of the alphaG275 residue is critical for activity | Vibrio harveyi |
G275I | site-directed mutagenesis, residue of the alpha-subunit, 15% of the wild-type activity, the torsional flexibility of the alphaG275 residue is critical for activity | Vibrio harveyi |
G275P | site-directed mutagenesis, residue of the alpha-subunit, 0.04% of the wild-type activity, the torsional flexibility of the alphaG275 residue is critical for activity | Vibrio harveyi |
G284P | site-directed mutagenesis, residue of the alpha-subunit, 1-2% of the wild-type activity | Vibrio harveyi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | stopped flow spectroscopy | Vibrio harveyi | |
0.0003 | - |
FMNH2 | recombinant wild-type enzyme, pH 7.0, 25°C | Vibrio harveyi | |
0.0004 | - |
decanal | recombinant mutant alphaF261S, pH 7.0, 25°C | Vibrio harveyi | |
0.0004 | - |
decanal | recombinant mutant alphaG275I, pH 7.0, 25°C | Vibrio harveyi | |
0.0006 | - |
FMNH2 | recombinant mutant alphaG284P, pH 7.0, 25°C | Vibrio harveyi | |
0.001 | - |
decanal | recombinant wild-type enzyme, pH 7.0, 25°C | Vibrio harveyi | |
0.0016 | - |
decanal | recombinant mutant alphaF261Y, pH 7.0, 25°C | Vibrio harveyi | |
0.0017 | - |
decanal | recombinant mutant alphaF261A, pH 7.0, 25°C | Vibrio harveyi | |
0.0018 | - |
decanal | recombinant mutant alphaG275P, pH 7.0, 25°C | Vibrio harveyi | |
0.0022 | - |
decanal | recombinant mutant alphaG275A, pH 7.0, 25°C | Vibrio harveyi | |
0.0022 | - |
FMNH2 | recombinant mutant alphaG275P, pH 7.0, 25°C | Vibrio harveyi | |
0.0027 | - |
decanal | recombinant mutant alphaG275F, pH 7.0, 25°C | Vibrio harveyi | |
0.0037 | - |
decanal | recombinant mutant alphaG284P, pH 7.0, 25°C | Vibrio harveyi | |
0.0046 | - |
decanal | recombinant mutant alphaF261D, pH 7.0, 25°C | Vibrio harveyi | |
0.0075 | - |
FMNH2 | recombinant mutant alphaF261D, pH 7.0, 25°C | Vibrio harveyi | |
0.0192 | - |
FMNH2 | recombinant mutant alphaF261Y, pH 7.0, 25°C | Vibrio harveyi | |
0.0281 | - |
FMNH2 | recombinant mutant alphaF261A, pH 7.0, 25°C | Vibrio harveyi | |
0.0358 | - |
FMNH2 | recombinant mutant alphaG275A, pH 7.0, 25°C | Vibrio harveyi | |
0.0369 | - |
FMNH2 | recombinant mutant alphaF261S, pH 7.0, 25°C | Vibrio harveyi | |
0.0411 | - |
FMNH2 | recombinant mutant alphaG275F, pH 7.0, 25°C | Vibrio harveyi | |
0.0584 | - |
FMNH2 | recombinant mutant alphaG275I, pH 7.0, 25°C | Vibrio harveyi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vibrio harveyi | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant enzymes from Escherichia coli strain JM109 to over 85% homogeneity | Vibrio harveyi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
decanal + FMNH2 + O2 | formation of a 4a-hydroperoxy-FMN intermediate II | Vibrio harveyi | decanoate + FMN + H2O + hn | - |
ir | |
RCHO + FMNH2 + O2 | formation of a 4a-hydroperoxy-FMN intermediate II | Vibrio harveyi | RCOOH + FMN + H2O + hn | - |
ir |
Subunits | Comment | Organism |
---|---|---|
More | functional roles of conserved residues in the protease-labile, unstructured loop of the alpha-subunit, formed by residues 257-291, the loop undergoed conformational changes during catalysis, overview | Vibrio harveyi |
Synonyms | Comment | Organism |
---|---|---|
bacterial luciferase | - |
Vibrio harveyi |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Vibrio harveyi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Vibrio harveyi |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMNH2 | - |
Vibrio harveyi |