Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.14.29 extracted from

  • Tang, W.; Pettersson, H.; Norlin, M.
    Involvement of the PI3K/Akt pathway in estrogen-mediated regulation of human CYP7B1: identification of CYP7B1 as a novel target for PI3K/Akt and MAPK signalling (2008), J. Steroid Biochem. Mol. Biol., 112, 63-73.
    View publication on PubMed
Search for more literature for 1.14.14.29

Activating Compound

Activating Compound Comment Organism Structure
estrogen overexpression of PI3K or Akt significantly increases estrogenic upregulation of CYP7B1 in Hep-G2 cells. Estrogen receptor interacts with the PI3K/Akt pathway without binding to the CYP7B1 gene. In HEK293 cells, CYP7B1 transcription is much less affected by Akt, indicating that the mechanism for upregulation of CYP7B1 is different in different cell types Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
LY294002 specific inhibitor of phosphatidylinositol 3-kinase, abolishes estrogen receptor-mediated upregulation of a CYP7B1 promoter-luciferase reporter in Hep-G2 cells but not in HEK-293 cells Homo sapiens
additional information overexpression of Akt, in the absence of estrogen stimulation, suppresses basal CYP7B1 promoter activity by about 75%. Overexpression of dominant-negative mutant Akt abolishes estrogen receptor-mediated stimulation of CYP7B1 in Hep-G2 cells Homo sapiens
PD98059 markedly suppresses basal CYP7B1 promoter activity, abolishes upregulation of CYP7B1 by estrogen receptor in Hep-G2 cells Homo sapiens
SP600125 markedly suppresses basal CYP7B1 promoter activity, abolishes upregulation of CYP7B1 by estrogen receptor in Hep-G2 cells but not in HEK-293 cells Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
HEK-293 cell
-
 Homo sapiens
-
Hep-G2 cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dehydroepiandrosterone + NADPH + H+ + O2
-
 Homo sapiens ? + NADP+ + H2O
-
 ?

Synonyms

Synonyms Comment Organism
CYP7B1
-
 Homo sapiens
Steroid hydroxylase
-
 Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Homo sapiens

Expression

Organism Comment Expression
Homo sapiens expression of CYP7B1 by estrogen receptor can be mediated via the PI3K/Akt signal pathway. Treatment with LY294002, a specific inhibitor of phosphatidylinositol 3-kinase PI3K, abolishes estrogen-receptor-mediated up-regulation of a CYP7B1 promoter-luciferase reporter down
Homo sapiens expression of CYP7B1 by estrogen receptor can be mediated via the PI3K/Akt signal pathway. Overexpression of PI3K or Akt significantly increases estrogenic up-regulation of CYP7B1 up

General Information

General Information Comment Organism
physiological function important role for CYP7B1 in cellular growth, particularly in connection with estrogenic signalling Homo sapiens