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Literature summary for 1.14.14.29 extracted from

  • Pettersson, H.; Holmberg, L.; Axelson, M.; Norlin, M.
    CYP7B1-mediated metabolism of dehydroepiandrosterone and alpha-androstane-3beta,17beta-diol - potential role(s) for estrogen signaling (2008), FEBS J., 275, 1778-1789.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
5alpha-Androstane-3beta,17beta-diol inhibits hydroxylation of dehydroepiandrosterone by approximately 60-70% when both steroids are present at equimolar concentrations. A 10fold higher concentration of 5alpha-androstane-3beta,17beta-diol than of dehydroepiandrosterone in the incubation mixture results in the suppression of dehydroepiandrosterone hydroxylation by 80%. In HEK293 cells, the suppressive effect of 5alpha-androstane-3beta,17beta-diol on dehydroepiandrosterone metabolism is statistically significant also at a 10fold lower concentration of 5alpha-androstane-3beta,17beta-diol than of dehydroepiandrosterone in the incubation mixture Homo sapiens
5alpha-Androstane-3beta,17beta-diol inhibits hydroxylation of dehydroepiandrosterone by approximately 60-70% when both steroids are present at equimolar concentrations. A 10fold higher concentration of 5alpha-androstane-3beta,17beta-diol than of dehydroepiandrosterone in the incubation mixture results in the suppression of dehydroepiandrosterone hydroxylation by 80% Sus scrofa
dehydroepiandrosterone inhibits hydroxylation of 5alpha-androstane-3beta,17beta-diol Sus scrofa
additional information no inhibition: testosterone, dehydroepiandrosterone sulfate Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.003
-
5alpha-Androstane-3beta,17beta-diol
-
Sus scrofa
0.005
-
dehydroepiandrosterone
-
Sus scrofa

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
Sus scrofa
-
-

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Sus scrofa
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
HEK-293 cell
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5alpha-androstane-3beta,17beta-diol + NADPH + H+ + O2
-
Homo sapiens ?
-
?
5alpha-androstane-3beta,17beta-diol + NADPH + H+ + O2
-
Sus scrofa ?
-
?
dehydroepiandrosterone + NADPH + H+ + o2
-
Homo sapiens 7alpha-hydroxy-dehydroepiandrosterone + NADP+ + H2O
-
?
dehydroepiandrosterone + NADPH + H+ + o2
-
Sus scrofa 7alpha-hydroxy-dehydroepiandrosterone + NADP+ + H2O
-
?

Synonyms

Synonyms Comment Organism
CYP7B1
-
Homo sapiens
CYP7B1
-
Sus scrofa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0166
-
5alpha-Androstane-3beta,17beta-diol
-
Sus scrofa
0.033
-
dehydroepiandrosterone
-
Sus scrofa

Cofactor

Cofactor Comment Organism Structure
cytochrome P450
-
Homo sapiens
cytochrome P450
-
Sus scrofa

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.006
-
5alpha-Androstane-3beta,17beta-diol inhibition of hydroxylation of dehydroepiandrosterone Sus scrofa
0.024
-
dehydroepiandrosterone
-
Sus scrofa