Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.14.23 extracted from

  • Nguyen, L.B.; Shefer, S.; Salen, G.; Chiang, J.Y.L.; Patel, M.
    Cholesterol 7alpha-hydroxylase activities from human and rat liver are modulated in vitro posttranslationally by phosphorylation/dephosphorylation (1996), Hepatology, 24, 1468-1474.
    View publication on PubMed
Search for more literature for 1.14.14.23

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Homo sapiens
expression in Escherichia coli Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
51000
-
 x * 51000, SDS-PAGE, expressed in Escherichia coli Homo sapiens
51000
-
 x * 51000, SDS-PAGE, expressed in Escherichia coli Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Rattus norvegicus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
side-chain modification enzyme activity decreases after dephosphorylation with alkaline phosphatase and increases after phosphorylation with cAMP-dependent protein kinase Homo sapiens
side-chain modification enzyme activity decreases after dephosphorylation with alkaline phosphatase and increases after phosphorylation with cAMP-dependent protein kinase Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00077
-
 after incubation with 1 unit alkaline phosphatase for 30 min Homo sapiens
0.00092
-
 after incubation with 1 unit alkaline phosphatase for 30 min Rattus norvegicus
0.00196
-
-
 Homo sapiens
0.00213
-
-
 Rattus norvegicus
0.00294
-
 after incubation with 5 units cAMP-dependent protein kinase for 30 min Homo sapiens
0.00315
-
 after incubation with 5 units cAMP-dependent protein kinase for 30 min Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cholesterol + [reduced NADPH-hemoprotein reductase] + O2
-
 Homo sapiens 7alpha-hydroxycholesterol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
cholesterol + [reduced NADPH-hemoprotein reductase] + O2
-
 Rattus norvegicus 7alpha-hydroxycholesterol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Subunits

Subunits Comment Organism
? x * 51000, SDS-PAGE, expressed in Escherichia coli Homo sapiens
? x * 51000, SDS-PAGE, expressed in Escherichia coli Rattus norvegicus