Cloned (Comment) | Organism |
---|---|
gene dszA, DNA and amino acid sequence determination and analysis, sequence comparisons | Rhodococcus erythropolis |
Crystallization (Comment) | Organism |
---|---|
purified enzyme, 4°C, hanging drop vapor diffusion method, mixing of 42 mg/ml protein in 1 mM Tris-HCl buffer, pH 8.0, with an equal volume of well solution containing 12% PEG 400, 0.2 M CaCl*, and 0.01 M HEPES buffer, pH 7.5, 1 week, X-ray diffraction structure determination and analysis | Rhodococcus erythropolis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | 37% inhibition at 1 mM | Rhodococcus erythropolis | |
2,2'-bipyridine | - |
Rhodococcus erythropolis | |
2,2-bipyridine | 62% inhibition at 1 mM | Rhodococcus erythropolis | |
8-Quinolinol | 88% inhibition at 1 mM | Rhodococcus erythropolis | |
Ca2+ | 81% inhibition at 1 mM | Rhodococcus erythropolis | |
Co2+ | 27% inhibition at 1 mM | Rhodococcus erythropolis | |
Cu2+ | complete inhibition at 1 mM | Rhodococcus erythropolis | |
EDTA | 49% inhibition at 1 mM | Rhodococcus erythropolis | |
Fe2+ | 32% inhibition at 1 mM | Rhodococcus erythropolis | |
Hg2+ | complete inhibition at 1 mM | Rhodococcus erythropolis | |
Li+ | 20% inhibition at 1 mM | Rhodococcus erythropolis | |
Mn2+ | 72% inhibition at 1 mM | Rhodococcus erythropolis | |
N-bromosuccinimide | 17% inhibition at 1 mM | Rhodococcus erythropolis | |
N-ethylmaleimide | 27% inhibition at 1 mM | Rhodococcus erythropolis | |
Ni2+ | 78% inhibition at 1 mM | Rhodococcus erythropolis | |
NO3- | 39% inhibition at 1 mM | Rhodococcus erythropolis | |
p-chloromercuribenzoic acid | 50% inhibition at 1 mM | Rhodococcus erythropolis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
5,5'-dithiobis(2-nitrobenzoic acid) | slightly activating at 0.1 mM | Rhodococcus erythropolis | |
Cd2+ | slightly activating at 1 mM | Rhodococcus erythropolis | |
additional information | no or poor effect by 1 mM of Al3+, Zn2+, Mg2+, semicarbazide, and NaF | Rhodococcus erythropolis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
2 * 50000, SDS-PAGE | Rhodococcus erythropolis |
97000 | - |
gel filtration | Rhodococcus erythropolis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dibenzothiophene-5,5-dioxide + 2 FMNH2 + O2 | Rhodococcus erythropolis | - |
2'-hydroxybiphenyl-2-sulfinate + 2 FMN + H2O | - |
? | |
dibenzothiophene-5,5-dioxide + 2 FMNH2 + O2 | Rhodococcus erythropolis D-1 | - |
2'-hydroxybiphenyl-2-sulfinate + 2 FMN + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus erythropolis | Q6WE15 | gene dszA | - |
Rhodococcus erythropolis D-1 | Q6WE15 | gene dszA | - |
Purification (Comment) | Organism |
---|---|
native enzyme 102fold to homogeneity by two different steps of anion exchange chromatography, followed by hydrophobic interaction chromatography, and gel filtration | Rhodococcus erythropolis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
807 | - |
purified enzyme, pH 7.0, 35°C | Rhodococcus erythropolis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dibenzothiophene-5,5-dioxide + 2 FMNH2 + O2 | - |
Rhodococcus erythropolis | 2'-hydroxybiphenyl-2-sulfinate + 2 FMN + H2O | - |
? | |
dibenzothiophene-5,5-dioxide + 2 FMNH2 + O2 | - |
Rhodococcus erythropolis D-1 | 2'-hydroxybiphenyl-2-sulfinate + 2 FMN + H2O | - |
? | |
dibenz[c,e][1,2] oxathiin 6-oxide + 2 FMNH2 + O2 | i.e. sultine, sultine is nonenzymatically hydrolyzed to form 2'-hydroxybiphenyl 2-sulfinic acid, it is also oxidized to sultone. Once sultone is nonenzymatically formed from sultine, it is immediately converted to 2,2'-dihydroxybiphenyl by DszA | Rhodococcus erythropolis | 2,2'-dihydroxybiphenyl + 2 FMN + H2O | - |
? | |
dibenz[c,e][1,2] oxathiin 6-oxide + 2 FMNH2 + O2 | i.e. sultine, sultine is nonenzymatically hydrolyzed to form 2'-hydroxybiphenyl 2-sulfinic acid, it is also oxidized to sultone. Once sultone is nonenzymatically formed from sultine, it is immediately converted to 2,2'-dihydroxybiphenyl by DszA | Rhodococcus erythropolis D-1 | 2,2'-dihydroxybiphenyl + 2 FMN + H2O | - |
? | |
dibenz[c,e][1,2]oxathiin 6,6-dioxide + 2 FMNH2 + O2 | i.e. sultone, sultine is nonenzymatically hydrolyzed to form 2'-hydroxybiphenyl 2-sulfinic acid, it is also oxidized to sultone. Once sultone is nonenzymatically formed from sultine, it is immediately converted to 2,2'-dihydroxybiphenyl by DszA | Rhodococcus erythropolis | 2,2'-dihydroxybiphenyl + 2 FMN + H2O | - |
? | |
dibenz[c,e][1,2]oxathiin 6,6-dioxide + 2 FMNH2 + O2 | i.e. sultone, sultine is nonenzymatically hydrolyzed to form 2'-hydroxybiphenyl 2-sulfinic acid, it is also oxidized to sultone. Once sultone is nonenzymatically formed from sultine, it is immediately converted to 2,2'-dihydroxybiphenyl by DszA | Rhodococcus erythropolis D-1 | 2,2'-dihydroxybiphenyl + 2 FMN + H2O | - |
? | |
additional information | no activity with dibenzothiophene and 2'-hydroxybiphenyl 2-sulfinic acid, substrate specificity, overview. DszA may recognize the sulfone moiety within the structure of DBT sulfone and sultone | Rhodococcus erythropolis | ? | - |
? | |
additional information | no activity with dibenzothiophene and 2'-hydroxybiphenyl 2-sulfinic acid, substrate specificity, overview. DszA may recognize the sulfone moiety within the structure of DBT sulfone and sultone | Rhodococcus erythropolis D-1 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 50000, SDS-PAGE | Rhodococcus erythropolis |
Synonyms | Comment | Organism |
---|---|---|
DBT sulfone monooxygenase | - |
Rhodococcus erythropolis |
dszA | - |
Rhodococcus erythropolis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Rhodococcus erythropolis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
purified enzyme, stable up to | Rhodococcus erythropolis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Rhodococcus erythropolis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 10 | purified enzyme, stable at | Rhodococcus erythropolis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMNH2 | essentially required | Rhodococcus erythropolis | |
additional information | NADH flavin reductase, essentially required | Rhodococcus erythropolis |
General Information | Comment | Organism |
---|---|---|
metabolism | enzyme DszA is involved in the microbial DBT desulfurization metabolism and catalyzes the conversion of dibenzothiophene sulfone to 2'-hydroxybiphenyl 2-sulfinic acid in the presence of flavin reductase with cleavage of the carbon-sulfur bond in the dibenzothiophene skeleton | Rhodococcus erythropolis |