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Literature summary for 1.14.14.21 extracted from

  • Izumi, Y.; Ohshiro, T.
    Purification and characterization of enzymes involved in desulfurization of dibezothiophene in fossil fuels (2001), J. Mol. Catal. B, 11, 1061-1064.
No PubMed abstract available

Crystallization (Commentary)

Crystallization (Comment) Organism
purified enzyme DszC, hanging drop vapour diffusion method, using a reservoir suliton containing 30% polyethylene glycol, 0.1 M sodium citrate, pH 5.6, and 0.2 M ammonium acetate, 4°C, X-ray diffraction structure determination and analysis Rhodococcus erythropolis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dibenzothiophene + 2 FMNH2 + 2 O2 Rhodococcus erythropolis
-
dibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O
-
?
dibenzothiophene + 2 FMNH2 + 2 O2 Rhodococcus erythropolis D-1
-
dibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O
-
?

Organism

Organism UniProt Comment Textmining
Rhodococcus erythropolis A0A0C6DRW4 gene dszC
-
Rhodococcus erythropolis D-1 A0A0C6DRW4 gene dszC
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme to homogeneity by hydrophobic interaction and anion exchange chromatography. and gel filtration Rhodococcus erythropolis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,8-dimethyldibenzothiophene + 2 FMNH2 + 2 O2 best substrate Rhodococcus erythropolis 2,8-dimethyldibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O
-
?
2,8-dimethyldibenzothiophene + 2 FMNH2 + 2 O2 best substrate Rhodococcus erythropolis D-1 2,8-dimethyldibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O
-
?
3,4-benzo-dibenzothiophene + 2 FMNH2 + 2 O2 low activity Rhodococcus erythropolis 3,4-benzo-dibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O
-
?
3,4-benzo-dibenzothiophene + 2 FMNH2 + 2 O2 low activity Rhodococcus erythropolis D-1 3,4-benzo-dibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O
-
?
4,6-dimethyldibenzothiophene + 2 FMNH2 + 2 O2
-
Rhodococcus erythropolis 4,6-dimethyldibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O
-
?
4,6-dimethyldibenzothiophene + 2 FMNH2 + 2 O2
-
Rhodococcus erythropolis D-1 4,6-dimethyldibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O
-
?
dibenzothiophene + 2 FMNH2 + 2 O2
-
Rhodococcus erythropolis dibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O
-
?
dibenzothiophene + 2 FMNH2 + 2 O2 high activity Rhodococcus erythropolis dibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O
-
?
dibenzothiophene + 2 FMNH2 + 2 O2
-
Rhodococcus erythropolis D-1 dibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O
-
?
dibenzothiophene + 2 FMNH2 + 2 O2 high activity Rhodococcus erythropolis D-1 dibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O
-
?
thioxanthen-9-one + 2 FMNH2 + 2 O2
-
Rhodococcus erythropolis ?
-
?

Synonyms

Synonyms Comment Organism
DBT monooxygenase
-
Rhodococcus erythropolis
dibenzothiophene monooxygenase
-
Rhodococcus erythropolis
dszC
-
Rhodococcus erythropolis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
Rhodococcus erythropolis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Rhodococcus erythropolis

General Information

General Information Comment Organism
metabolism the enzyme is involved in the pathway of microbial dibenzothiophene desulfurization, overview Rhodococcus erythropolis