Cloned (Comment) | Organism |
---|---|
gene dszC, recombinant expression in Escherichia coli strain BL21(DE3) | Rhodococcus sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
6 * 45000, about, recombinant enzyme, SDS-PAGE | Rhodococcus sp. |
294000 | - |
recombinant enzyme, gel filtration | Rhodococcus sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dibenzothiophene + 2 FMNH2 + 2 O2 | Rhodococcus sp. | - |
dibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
dibenzothiophene + 2 FMNH2 + 2 O2 | Rhodococcus sp. IIPS7 | - |
dibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus sp. | Q3HSE4 | gene dszC | - |
Rhodococcus sp. IIPS7 | Q3HSE4 | gene dszC | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme 39fold from Escherichia coli strain BL21(DE3) by affinity chromatography | Rhodococcus sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3 | 8 | purified recombinant enzyme, pH 7.0, 30°C | Rhodococcus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dibenzothiophene + 2 FMNH2 + 2 O2 | - |
Rhodococcus sp. | dibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
dibenzothiophene + 2 FMNH2 + 2 O2 | - |
Rhodococcus sp. IIPS7 | dibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
homohexamer | 6 * 45000, about, recombinant enzyme, SDS-PAGE | Rhodococcus sp. |
Synonyms | Comment | Organism |
---|---|---|
DBT monooxygenase | - |
Rhodococcus sp. |
dibenzothiophene monooxygenase | - |
Rhodococcus sp. |
dszC | - |
Rhodococcus sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Rhodococcus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Rhodococcus sp. |
General Information | Comment | Organism |
---|---|---|
metabolism | dibenzothiophene monooxygenase is the first enzyme involved in the degradation of dibenzothiophene | Rhodococcus sp. |