Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Leptospira interrogans serovar Icterohaemorrhagiae |
Crystallization (Comment) | Organism |
---|---|
heme oxygenase-heme complex at 1.73 A resolution. A hydrogen-bonded network of structural water molecules that extends from the catalytic site to the protein surface is observed. A long-range communication exists between the outer fringes of the hydrogen-bonded network of structural waters and the heme active site during catalysis. Model for the productive association of ferredoxin-NADP+ reductase and heme oxygenase | Leptospira interrogans serovar Icterohaemorrhagiae |
Protein Variants | Comment | Organism |
---|---|---|
F157A | mutant is unable to carry out the complete degradation of heme to biliverdin, the reaction is arrested at the verdoheme stage. The protein displays bands at 357, 525 and 678 nm, reminiscent of the absorption spectrum reported for the HO-verdoheme complex. Overexpression of the HO F157 variants causes the Escherichia coli cells to turn dark green color | Leptospira interrogans serovar Icterohaemorrhagiae |
F157I | mutant is unable to carry out the complete degradation of heme to biliverdin, the reaction is arrested at the verdoheme stage. The protein displays bands at 357, 525 and 678 nm, reminiscent of the absorption spectrum reported for the HO-verdoheme complex. Overexpression of the HO F157 variants causes the Escherichia coli cells to turn dark green color | Leptospira interrogans serovar Icterohaemorrhagiae |
additional information | a C26S variant lacking the last 20 C-terminal residues incorporates the prosthetic group efficiently and is active | Leptospira interrogans serovar Icterohaemorrhagiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00177 | - |
[reduced NADPH-hemoprotein reductase] | wild-type, pH 7.5, 25°C | Leptospira interrogans serovar Icterohaemorrhagiae | |
0.00271 | - |
[reduced NADPH-hemoprotein reductase] | mutant F157I, pH 7.5, 25°C | Leptospira interrogans serovar Icterohaemorrhagiae |
Organism | UniProt | Comment | Textmining |
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Leptospira interrogans serovar Icterohaemorrhagiae | - |
- |
- |
Leptospira interrogans serovar Icterohaemorrhagiae serovar Lai 56601 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
heme + [reduced NADPH-hemoprotein reductase] + O2 | - |
Leptospira interrogans serovar Icterohaemorrhagiae | biliverdin + Fe2+ + CO + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
heme + [reduced NADPH-hemoprotein reductase] + O2 | - |
Leptospira interrogans serovar Icterohaemorrhagiae serovar Lai 56601 | biliverdin + Fe2+ + CO + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |