Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.14.18 extracted from

  • Sigala, P.A.; Morante, K.; Tsumoto, K.; Caaveiro, J.M.; Goldberg, D.E.
    In-cell enzymology to probe His-heme ligation in heme oxygenase catalysis (2016), Biochemistry, 55, 4836-4849 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.14.18

Application

Application Comment Organism
analysis live-cell activity assay based on heterologous coexpression in Escherichia coli of heme oxygenase and its mutants and a fluorescent biliverdin biosensor Homo sapiens
analysis live-cell activity assay based on heterologous coexpression in Escherichia coli of heme oxygenase and its mutants and a fluorescent biliverdin biosensor Synechocystis sp. PCC 6803

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Homo sapiens
expression in Escherichia coli Synechocystis sp. PCC 6803

Crystallization (Commentary)

Crystallization (Comment) Organism
mutant H25R with bound heme, to 2.95 A resolution. Contrary to wild-type, the Arg25 side-chain in the H25R mutant is oriented away from the parent His25 position, accompanied by reorientation of the Glu29 side-chain Homo sapiens
mutant H25R with bound heme, to 2.95 A resolution. Contrary to wild-type, the Arg25 side-chain in the H25R mutant is oriented away from the parent His25 position, accompanied by reorientation of the Glu29 side-chain Synechocystis sp. PCC 6803

Protein Variants

Protein Variants Comment Organism
H17A mutant retains activity in live-cell assay Synechocystis sp. PCC 6803
H17C mutant retains activity in live-cell assay Synechocystis sp. PCC 6803
H17E very poor or no activity in live-cell assay Synechocystis sp. PCC 6803
H17F very poor or no activity in live-cell assay, presence of heme, no binding of biliverdin observed Synechocystis sp. PCC 6803
H17K mutant retains activity in live-cell assay, and binds biliverdin Synechocystis sp. PCC 6803
H17M mutant retains activity and binds biliverdin Synechocystis sp. PCC 6803
H17Q mutant retains activity in live-cell assay Synechocystis sp. PCC 6803
H17R mutant retains activity in live-cell assay, and binds biliverdin. No activity in vitro Synechocystis sp. PCC 6803
H17Y very poor or no activity in live-cell assay Synechocystis sp. PCC 6803
H25M/E29A mutant retains activity Homo sapiens
H25R crystallization analysis Homo sapiens
H25R/E29A mutant retains activity Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P09601
-
-
Synechocystis sp. PCC 6803 P72849
-
-

Synonyms

Synonyms Comment Organism
heme oxygenase 1
-
 Synechocystis sp. PCC 6803
Hmox1
-
 Homo sapiens
Ho1
-
 Homo sapiens
pbsA1
-
 Synechocystis sp. PCC 6803