Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapour diffusion method using 12-15% (w/v) polyethylene glycol 3350, 0.15-0.20 M magnesium formate, and 10-20 mM NAD | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
H193N | inactive enzyme | Escherichia coli |
H73A | no spectrum compared with native ChuS | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography and Resource Q column chromatography | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
heme + NADH + O2 | - |
Escherichia coli | biliverdin + Fe2+ + CO + NAD+ + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ChuS | - |
Escherichia coli |