Literature summary for 1.14.14.176 extracted from

Biggs, B.W.; Rouck, J.E.; Kambalyal, A.; Arnold, W.; Lim, C.G.; De Mey, M.; ONeil-Johnson, M.; Starks, C.M.; Das, A.; Ajikumar, P.K.
Orthogonal assays clarify the oxidative biochemistry of taxol P450 CYP725A4 (2016), ACS Chem. Biol., 11, 1445-1451 .

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Taxus brevifolia

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
taxa-4,11-diene + [reduced NADPH-hemoprotein reductase] + O2	Taxus brevifolia	-	taxa-4(20),11-dien-5alpha-ol + [oxidized NADPH-hemoprotein reductase] + H2O	-	?

Organism

Organism	UniProt	Comment	Textmining
Taxus brevifolia	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA column chromatography	Taxus brevifolia

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
taxa-4,11-diene + [reduced NADPH-hemoprotein reductase] + O2	-	Taxus brevifolia	taxa-4(20),11-dien-5alpha-ol + [oxidized NADPH-hemoprotein reductase] + H2O	-	?

Synonyms

Synonyms	Comment	Organism
CYP725A4	-	Taxus brevifolia
taxa-4(5),11(12)-diene 5-hydroxylase	-	Taxus brevifolia

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Release 2023.1 (January 2023)