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Literature summary for 1.14.14.175 extracted from

  • Ma, Y.; Ma, X.H.; Meng, F.Y.; Zhan, Z.L.; Guo, J.; Huang, L.Q.
    RNA interference targeting CYP76AH1 in hairy roots of Salvia miltiorrhiza reveals its key role in the biosynthetic pathway of tanshinones (2016), Biochem. Biophys. Res. Commun., 477, 155-160 .
    View publication on PubMed
Search for more literature for 1.14.14.175

Protein Variants

Protein Variants Comment Organism
additional information RNAi-mediated silencing of CYP76AH1 in hairy roots, construction of a CYP76AH1 RNAi system for hairy roots using transformation via Agrobacterium rhizogenes strain C58C1. Metabolic analysis of RNAi-AH1 hairy root lines shows a significantly increased accumulation of miltiradiene and decreased concentration of ferruginol compared to the control lines. The content of tanshinones decreases significantly after silencing of CYP76AH1 Salvia miltiorrhiza

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
abieta-8,11,13-triene + [reduced NADPH-hemoprotein reductase] + O2 Salvia miltiorrhiza
-
 ferruginol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Salvia miltiorrhiza S4UX02
-
-

Source Tissue

Source Tissue Comment Organism Textmining
hairy root
-
 Salvia miltiorrhiza
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
abieta-8,11,13-triene + [reduced NADPH-hemoprotein reductase] + O2
-
 Salvia miltiorrhiza ferruginol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
abieta-8,11,13-triene + [reduced NADPH-hemoprotein reductase] + O2 i.e. miltiradiene Salvia miltiorrhiza ferruginol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Synonyms

Synonyms Comment Organism
CYP76AH1
-
 Salvia miltiorrhiza

Cofactor

Cofactor Comment Organism Structure
cytochrome P-450
-
 Salvia miltiorrhiza
NADPH
-
 Salvia miltiorrhiza

General Information

General Information Comment Organism
metabolism the enzyme catalyzes the first committed step in the biosynthesis of tanshinones, pathay overview Salvia miltiorrhiza
physiological function CYP76AH1 catalyzes the conversion of the carbon skeleton miltiradiene into the intermediate ferruginol, which is involved in the biosynthesis of tanshinones, the chief bioactive ingredients of Salvia miltiorrhiza Salvia miltiorrhiza