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Literature summary for 1.14.14.17 extracted from
- Sterol biosynthesis by a prokaryote: first in vitro identification of the genes encoding squalene epoxidase and lanosterol synthase from Methylococcus capsulatus (2007), Biosci. Biotechnol. Biochem., 71, 2543-2550.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Triton X-100 | activates at 0.1% | Methylococcus capsulatus |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determination and analysis, functional expression in Escherichia coli strain BL21(DE3) | Methylococcus capsulatus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| squalene + reduced cytochrome P450 + O2 | Methylococcus capsulatus | first step in the cyclic chloesterol biosynthesis | (S)-squalene-2,3-epoxide + cytochrome P450 + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methylococcus capsulatus | Q603D5 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| squalene + reduced cytochrome P450 + H+ + O2 | absolutely stereo- and regiospecific reaction, co-reaction with NADPH-cytochrome P450 reductase | Methylococcus capsulatus | (3S)-squalene-2,3-epoxide + cytochrome P450 + H2O | - |
? | |
| squalene + reduced cytochrome P450 + O2 | first step in the cyclic chloesterol biosynthesis | Methylococcus capsulatus | (S)-squalene-2,3-epoxide + cytochrome P450 + H2O | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| squalene epoxidase | - |
Methylococcus capsulatus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Methylococcus capsulatus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Methylococcus capsulatus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Methylococcus capsulatus | |
| reduced cytochrome P450 | - |
Methylococcus capsulatus |
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Release 2023.1 (January 2023)
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