KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00043 | - |
FAD | - |
Rattus norvegicus | |
0.00085 | - |
1-carba-1-deaza-FAD | - |
Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
squalene + electron donor + O2 | Rattus norvegicus | first oxygenase and last nonsterol reaction of sterol biosynthesis | (S)-squalene-2,3-epoxide + oxidized electron donor + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(3S)-2,3-epoxy-2,3-dihydrosqualene + 1-carba-1-deaza-FAD + H2O | - |
Rattus norvegicus | squalene + 1-carba-1-deaza-FADH2 + O2 | - |
r | |
(3S)-2,3-epoxy-2,3-dihydrosqualene + FAD + H2O | - |
Rattus norvegicus | squalene + FADH2 + O2 | - |
r | |
squalene + electron donor + O2 | first oxygenase and last nonsterol reaction of sterol biosynthesis | Rattus norvegicus | (S)-squalene-2,3-epoxide + oxidized electron donor + H2O | - |
? | |
squalene + NAD(P)H + O2 | - |
Rattus norvegicus | (S)-squalene-2,3-epoxide + NADP+ + H2O | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
1-Carba-1-deazaFAD | can replace FAD as cofactor | Rattus norvegicus | |
FAD | flavoprotein | Rattus norvegicus | |
additional information | 5-carba-5-deazaFAD cannot replace FAD as cofactor | Rattus norvegicus |