Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | reconstitution of squalene epoxidase activity by addition of: NADPH-cytochrome P-450 reductase, EC 1.6.2.4, FAD and Triton X-100 | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Rattus norvegicus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47000 | - |
x * 47000, SDS-PAGE | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE-cellulose, alumina gel, hydroxylapatite, CM-Sephadex C-50, Blue Sepharose 4B | Rattus norvegicus |
Renatured (Comment) | Organism |
---|---|
reconstitution of purified enzyme with the addition of NADPH-cytochrome P450 reductase, FAD and Triton X-100 | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00278 | - |
- |
Rattus norvegicus |
Subunits | Comment | Organism |
---|---|---|
? | x * 47000, SDS-PAGE | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | flavoprotein | Rattus norvegicus | |
FAD | required for activity | Rattus norvegicus |