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Literature summary for 1.14.14.166 extracted from

  • Dang, T.T.; Facchini, P.J.
    CYP82Y1 is N-methylcanadine 1-hydroxylase, a key noscapine biosynthetic enzyme in opium poppy (2014), J. Biol. Chem., 289, 2013-2026 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.14.166

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-N-methylcanadine + [reduced NADPH-hemoprotein reductase] + O2 Papaver somniferum the enzyme participates in the biosynthesis of the isoquinoline alkaloid noscapine (S)-1-hydroxy-N-methylcanadine + [oxidized NADPH-hemoprotein reductase] + H2O
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 ?

Organism

Organism UniProt Comment Textmining
Papaver somniferum I3PLR1
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Source Tissue

Source Tissue Comment Organism Textmining
flower bud occurrence of CYP82Y1 transcripts in all plant organs, with the highest levels detected in stems Papaver somniferum
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leaf occurrence of CYP82Y1 transcripts in all plant organs, with the highest levels detected in stems Papaver somniferum
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root occurrence of CYP82Y1 transcripts in all plant organs, with the highest levels detected in stems Papaver somniferum
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stem occurrence of CYP82Y1 transcripts in all plant organs, with the highest levels detected in stems Papaver somniferum
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-N-methylcanadine + [reduced NADPH-hemoprotein reductase] + O2
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 Papaver somniferum (S)-1-hydroxy-N-methylcanadine + [oxidized NADPH-hemoprotein reductase] + H2O
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 ?
(S)-N-methylcanadine + [reduced NADPH-hemoprotein reductase] + O2 the enzyme participates in the biosynthesis of the isoquinoline alkaloid noscapine Papaver somniferum (S)-1-hydroxy-N-methylcanadine + [oxidized NADPH-hemoprotein reductase] + H2O
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 ?

Synonyms

Synonyms Comment Organism
CYP82Y1
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 Papaver somniferum
N-methylcanadine 1-hydroxylase
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 Papaver somniferum

Cofactor

Cofactor Comment Organism Structure
cytochrome P-450 heme-thiolate enzyme Papaver somniferum

General Information

General Information Comment Organism
malfunction suppression of CYP82Y1 using virus-induced gene silencing causes a significant reduction in the levels of noscapine, narcotoline, and a putative downstream secoberbine intermediate and also results in increased accumulation of the upstream pathway intermediates scoulerine, tetrahydrocolumbamine, canadine, and N-methylcanadine Papaver somniferum
metabolism the enzyme participates in the biosynthesis of the isoquinoline alkaloid noscapine Papaver somniferum