Cloned (Comment) | Organism |
---|---|
expression in Saccharomyces cerevisiae. Owing to difficulties with heterologous expression in yeast, CYP82X2 is synthesized with the first 60 N-terminal amino acids replaced with the first 43 N-terminal amino acids from the lettuce cytochrome P450 germacrene A oxidase | Papaver somniferum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0178 | - |
(S)-1-hydroxy-N-methylcanadine | pH 7.5, 30°C | Papaver somniferum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-1-hydroxy-N-methylcanadine + [reduced NADPH-hemoprotein reductase] + O2 | Papaver somniferum | the enzyme participates in the biosynthesis of the isoquinoline alkaloid noscapine | (13S,14R)-1,13-dihydroxy-N-methylcanadine + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Papaver somniferum | I3PLR0 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Papaver somniferum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
stem | - |
Papaver somniferum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-1-hydroxy-N-methylcanadine + [reduced NADPH-hemoprotein reductase] + O2 | the enzyme participates in the biosynthesis of the isoquinoline alkaloid noscapine | Papaver somniferum | (13S,14R)-1,13-dihydroxy-N-methylcanadine + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
(S)-1-hydroxy-N-methylcanadine + [reduced NADPH-hemoprotein reductase] + O2 | the enzyme shows strict substrate specificity | Papaver somniferum | (13S,14R)-1,13-dihydroxy-N-methylcanadine + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CYP82X2 | - |
Papaver somniferum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Papaver somniferum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Papaver somniferum |
General Information | Comment | Organism |
---|---|---|
malfunction | CYP719A21, CYP82X1 and CXE1 showed reduced transcript levels in CYP82X2-silenced plants | Papaver somniferum |
metabolism | the enzyme participates in the biosynthesis of the isoquinoline alkaloid noscapine | Papaver somniferum |