Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.14.162 extracted from

  • Otani, K.; Takahashi, T.; Furuya, T.; Ayabe, S.
    Licodione synthase, a cytochrome P450 monooxygenase catalyzing 2-hydroxylation of 5-deoxyflavanone, in cultured Glycyrrhiza echinata L. cells (1994), Plant Physiol., 105, 1427-1432.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
additional information yeast extract induces LS activity Glycyrrhiza echinata

Inhibitors

Inhibitors Comment Organism Structure
Ancymidol 0.001 mM: 33% inhibition, 0.01 mM: 68% inhibition, 0.1 mM: 81% inhibition Glycyrrhiza echinata
ketoconazole 0.001 mM: 44% inhibition, 0.01 mM: 86% inhibition, 0.1 mM: 72% inhibition Glycyrrhiza echinata
Metyrapone 0.001 mM: 23% inhibition, 0.01 mM: 57% inhibition, 0.1 mM: 74% inhibition Glycyrrhiza echinata

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
Glycyrrhiza echinata
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Glycyrrhiza echinata involved in flavonoid biosynthesis, may play a role symbiotic plant-microbe interactions ?
-
?

Organism

Organism UniProt Comment Textmining
Glycyrrhiza echinata
-
-
-

Reaction

Reaction Comment Organism Reaction ID
a flavanone + [reduced NADPH-hemoprotein reductase] + O2 = a 2-hydroxyflavanone + [oxidized NADPH-hemoprotein reductase] + H2O a heme-thiolate protein, P-450, it probably forms 2-hydroxyliquiritigenin which spontaneously forms licodione, NADH can act instead of NADPH, but more slowly, reaction mechanism Glycyrrhiza echinata

Source Tissue

Source Tissue Comment Organism Textmining
cell suspension culture yeast extract-induced Glycyrrhiza echinata
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2S)-liquiritigenin + [reduced NADPH-hemoprotein reductase] + O2 requires NAD(P)H and O2 for activity, the reaction mechanism is likely to be 2-hydroxylation of the flavanone molecule to yield 2-hydroxyliquiritigenin and the subsequent hemiacetal opening to give licodione, introduces one atom of oxygen into the substrate, NADH serves as hydride donor less effectively than NADPH Glycyrrhiza echinata licodione + [oxidized NADPH-hemoprotein reductase] + H2O i.e. 1-(2,4-dihydroxyphenyl)-3-(4-hydroxyphenyl)-1,3-propanedione ?
(2S)-liquiritigenin + [reduced NADPH-hemoprotein reductase] + O2 requires NAD(P)H and O2 for activity, the reaction mechanism is likely to be 2-hydroxylation of the flavanone molecule to yield 2-hydroxyliquiritigenin and the subsequent hemiacetal opening to give licodione, introduces one atom of oxygen into the substrate, NADPH serves as hydride donor more effectively than NADH Glycyrrhiza echinata licodione + [oxidized NADPH-hemoprotein reductase] + H2O i.e. 1-(2,4-dihydroxyphenyl)-3-(4-hydroxyphenyl)-1,3-propanedione ?
(2S)-naringenin + [reduced NADPH-hemoprotein reductase] + O2
-
Glycyrrhiza echinata 2-hydroxynaringenin + [oxidized NADPH-hemoprotein reductase] + H2O the product is further converted into apigenin when treated with acid ?
additional information involved in flavonoid biosynthesis, may play a role symbiotic plant-microbe interactions Glycyrrhiza echinata ?
-
?

Synonyms

Synonyms Comment Organism
LS
-
Glycyrrhiza echinata
More LS is likely to be identical with flavone synthase II from soybean Glycyrrhiza echinata

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Glycyrrhiza echinata

Cofactor

Cofactor Comment Organism Structure
cytochrome P450 cytochrome P450-dependent, cytochrome P450 monooxygenase Glycyrrhiza echinata