Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | yeast extract induces LS activity | Glycyrrhiza echinata |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ancymidol | 0.001 mM: 33% inhibition, 0.01 mM: 68% inhibition, 0.1 mM: 81% inhibition | Glycyrrhiza echinata | |
ketoconazole | 0.001 mM: 44% inhibition, 0.01 mM: 86% inhibition, 0.1 mM: 72% inhibition | Glycyrrhiza echinata | |
Metyrapone | 0.001 mM: 23% inhibition, 0.01 mM: 57% inhibition, 0.1 mM: 74% inhibition | Glycyrrhiza echinata |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Glycyrrhiza echinata | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Glycyrrhiza echinata | involved in flavonoid biosynthesis, may play a role symbiotic plant-microbe interactions | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Glycyrrhiza echinata | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
a flavanone + [reduced NADPH-hemoprotein reductase] + O2 = a 2-hydroxyflavanone + [oxidized NADPH-hemoprotein reductase] + H2O | a heme-thiolate protein, P-450, it probably forms 2-hydroxyliquiritigenin which spontaneously forms licodione, NADH can act instead of NADPH, but more slowly, reaction mechanism | Glycyrrhiza echinata |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell suspension culture | yeast extract-induced | Glycyrrhiza echinata | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2S)-liquiritigenin + [reduced NADPH-hemoprotein reductase] + O2 | requires NAD(P)H and O2 for activity, the reaction mechanism is likely to be 2-hydroxylation of the flavanone molecule to yield 2-hydroxyliquiritigenin and the subsequent hemiacetal opening to give licodione, introduces one atom of oxygen into the substrate, NADH serves as hydride donor less effectively than NADPH | Glycyrrhiza echinata | licodione + [oxidized NADPH-hemoprotein reductase] + H2O | i.e. 1-(2,4-dihydroxyphenyl)-3-(4-hydroxyphenyl)-1,3-propanedione | ? | |
(2S)-liquiritigenin + [reduced NADPH-hemoprotein reductase] + O2 | requires NAD(P)H and O2 for activity, the reaction mechanism is likely to be 2-hydroxylation of the flavanone molecule to yield 2-hydroxyliquiritigenin and the subsequent hemiacetal opening to give licodione, introduces one atom of oxygen into the substrate, NADPH serves as hydride donor more effectively than NADH | Glycyrrhiza echinata | licodione + [oxidized NADPH-hemoprotein reductase] + H2O | i.e. 1-(2,4-dihydroxyphenyl)-3-(4-hydroxyphenyl)-1,3-propanedione | ? | |
(2S)-naringenin + [reduced NADPH-hemoprotein reductase] + O2 | - |
Glycyrrhiza echinata | 2-hydroxynaringenin + [oxidized NADPH-hemoprotein reductase] + H2O | the product is further converted into apigenin when treated with acid | ? | |
additional information | involved in flavonoid biosynthesis, may play a role symbiotic plant-microbe interactions | Glycyrrhiza echinata | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
LS | - |
Glycyrrhiza echinata |
More | LS is likely to be identical with flavone synthase II from soybean | Glycyrrhiza echinata |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Glycyrrhiza echinata |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome P450 | cytochrome P450-dependent, cytochrome P450 monooxygenase | Glycyrrhiza echinata |