Cloned (Comment) | Organism |
---|---|
expression of the gene in Saccharomyces cerevisiae confirms its function as an authentic 7-deoxyloganetic acid synthase that catalyzes the 3 step oxidation of iridodial-nepetalactol to form 7-deoxyloganetic acid | Catharanthus roseus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(+)-cis,trans-nepetalactol + 3 [reduced NADPH-hemoprotein reductase] + 3 O2 | Catharanthus roseus | the enzyme catalyses three successive reactions leading to biosynthesis of monoterpenoid indole alkaloids, overall reaction | 7-deoxyloganetate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O | - |
? | |
(+)-cis,trans-nepetalactol + 3 [reduced NADPH-hemoprotein reductase] + 3 O2 | Catharanthus roseus cv. Little Delicata | the enzyme catalyses three successive reactions leading to biosynthesis of monoterpenoid indole alkaloids, overall reaction | 7-deoxyloganetate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Catharanthus roseus | W8JIS5 | - |
- |
Catharanthus roseus cv. Little Delicata | W8JIS5 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(+)-cis,trans-nepetalactol + 3 [reduced NADPH-hemoprotein reductase] + 3 O2 | the enzyme catalyses three successive reactions leading to biosynthesis of monoterpenoid indole alkaloids, overall reaction | Catharanthus roseus | 7-deoxyloganetate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O | - |
? | |
(+)-cis,trans-nepetalactol + 3 [reduced NADPH-hemoprotein reductase] + 3 O2 | the enzyme catalyses three successive reactions leading to biosynthesis of monoterpenoid indole alkaloids, overall reaction | Catharanthus roseus cv. Little Delicata | 7-deoxyloganetate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O | - |
? | |
(+)-cis,trans-nepetalactol + [reduced NADPH-hemoprotein reductase] + O2 | the enzyme catalyses three successive reactions leading to biosynthesis of monoterpenoid indole alkaloids | Catharanthus roseus | 7-deoxyloganetic alcohol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
(+)-cis,trans-nepetalactol + [reduced NADPH-hemoprotein reductase] + O2 | the enzyme catalyses three successive reactions leading to biosynthesis of monoterpenoid indole alkaloids | Catharanthus roseus cv. Little Delicata | 7-deoxyloganetic alcohol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
7-deoxyloganetic alcohol + [reduced NADPH-hemoprotein reductase] + O2 | the enzyme catalyses three successive reactions leading to biosynthesis of monoterpenoid indole alkaloids | Catharanthus roseus | iridotrial + [oxidized NADPH-hemoprotein reductase] + 2 H2O | - |
? | |
7-deoxyloganetic alcohol + [reduced NADPH-hemoprotein reductase] + O2 | the enzyme catalyses three successive reactions leading to biosynthesis of monoterpenoid indole alkaloids | Catharanthus roseus cv. Little Delicata | iridotrial + [oxidized NADPH-hemoprotein reductase] + 2 H2O | - |
? | |
iridotrial + [reduced NADPH-hemoprotein reductase] + O2 | the enzyme catalyses three successive reactions leading to biosynthesis of monoterpenoid indole alkaloids | Catharanthus roseus | 7-deoxyloganetate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
iridotrial + [reduced NADPH-hemoprotein reductase] + O2 | the enzyme catalyses three successive reactions leading to biosynthesis of monoterpenoid indole alkaloids | Catharanthus roseus cv. Little Delicata | 7-deoxyloganetate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
7-deoxyloganetic acid synthase | - |
Catharanthus roseus |
7DLS | - |
Catharanthus roseus |
CYP76A26 | - |
Catharanthus roseus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome P-450 | a cytochrome P-450 (heme thiolate) enzyme | Catharanthus roseus |
General Information | Comment | Organism |
---|---|---|
malfunction | virus-induced gene silencing of 7-deoxyloganetic acid synthase in Catharanthus roseus greatly decreases levels of secologanin and the major monoterpenoid indole alkaloids, catharanthine and vindoline in silenced leaves | Catharanthus roseus |
metabolism | the enzyme catalyses three successive reactions in the seco-iridoid pathway leading to biosynthesis of monoterpenoid indole alkaloids | Catharanthus roseus |