Application | Comment | Organism |
---|---|---|
medicine | localization of mutations leading to congenital adrenal hyperplasia within the protein. The severity of the congenital adrenal hyperplasia clinical manifestations can be directly correlated with the degree of mutation-induced damage in terms of protein fold stability and active site changes in the structural model. The nonclassical phenotype is typically associated with mutations that have a compensatory effect, i.e. H-bonding replacing hydrophobic interactions and vice versa | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
modeling of mutant structures connected with congenital adrenal hyperplasia | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
G65E | 0.08% of wild-type activity with substrate progesterone | Homo sapiens |
T296N | 0.05% of wild-type activity with substrate progesterone | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00021 | - |
progesterone | wild-type, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
0.00046 | - |
17alpha-hydroxyprogesterone | mutant T296N, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
0.0011 | - |
progesterone | mutant T296N, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
0.0015 | - |
17alpha-hydroxyprogesterone | wild-type, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
0.0055 | - |
progesterone | mutant G65E, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
0.013 | - |
17alpha-hydroxyprogesterone | mutant G65E, pH not specified in the publication, temperature not specified in the publication | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P08686 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
17alpha-hydroxyprogesterone + NADPH + H+ + O2 | - |
Homo sapiens | 17,21-dihydroxyprogesterone + NADP+ + H2O | - |
? | |
progesterone + NADPH + H+ + O2 | - |
Homo sapiens | 11-deoxycorticosterone + NADP+ + H2O | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0077 | - |
progesterone | mutant T296N, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
0.032 | - |
17alpha-hydroxyprogesterone | mutant G65E, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
0.045 | - |
17alpha-hydroxyprogesterone | mutant T296N, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
0.062 | - |
progesterone | mutant G65E, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
2.8 | - |
progesterone | wild-type, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
4 | - |
17alpha-hydroxyprogesterone | wild-type, pH not specified in the publication, temperature not specified in the publication | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.3 | - |
17alpha-hydroxyprogesterone | mutant G65E, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
7 | - |
progesterone | mutant T296N, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
11.2 | - |
progesterone | mutant G65E, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
100 | - |
17alpha-hydroxyprogesterone | mutant T296N, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
2667 | - |
17alpha-hydroxyprogesterone | wild-type, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
13333 | - |
progesterone | wild-type, pH not specified in the publication, temperature not specified in the publication | Homo sapiens |