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Literature summary for 1.14.14.16 extracted from

  • Yoshimoto, F.K.; Zhou, Y.; Peng, H.M.; Stidd, D.; Yoshimoto, J.A.; Sharma, K.K.; Matthew, S.; Auchus, R.J.
    Minor activities and transition state properties of the human steroid hydroxylases cytochromes P450c17 and P450c21, from reactions observed with deuterium-labeled substrates (2012), Biochemistry, 51, 7064-7077.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.14.16

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutant enzymes in yeast microsomes Homo sapiens

Protein Variants

Protein Variants Comment Organism
V359A site-directed mutagenesis, the mutant shows altered product formation compared to the wild-type enzyme Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information calculation of intramolecular and intermolecular kinetic isotope effects for wild-type and mutant enzymes with substrates 21-[2H]-progesterone, 21,21,21-[2H3]-progesterone, and 21,21,21-[2H3]-17-hydroxyprogesterone, overview Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
 Homo sapiens
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
17alpha-hydroxyprogesterone + [reduced NADPH-hemoprotein reductase] + O2 Homo sapiens
-
 11-deoxycortisol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
progesterone + [reduced NADPH-hemoprotein reductase] + O2 Homo sapiens
-
 11-deoxycorticosterone + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type and mutant enzymes from yeast microsomes Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
17alpha-hydroxyprogesterone + [reduced NADPH-hemoprotein reductase] + O2
-
 Homo sapiens 11-deoxycortisol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
additional information human CYP21A2 also has progesterone 16alpha-hydroxylase activity. Product analysis by LC-MS/MS Homo sapiens ?
-
 ?
progesterone + [reduced NADPH-hemoprotein reductase] + O2
-
 Homo sapiens 11-deoxycorticosterone + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
progesterone + [reduced NADPH-hemoprotein reductase] + O2 mutant V359A produces an additional metabolite, 16alpha-hydroxyprogesterone of progesterone, very low activity with the wild-type enzyme Homo sapiens 16alpha-hydroxy-progesterone + [oxidized NADPH-hemoprotein reductase] + H2O product identification by TLC ?

Synonyms

Synonyms Comment Organism
P450c21
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Homo sapiens