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Literature summary for 1.14.14.16 extracted from

  • Sushko, T.A.; Gilep, A.A.; Usanov, S.A.
    Mechanism of intermolecular interactions of microsomal cytochrome P450s CYP17 and CYP21 involved in steroid hormone biosynthesis (2012), Biochemistry (Moscow), 77, 585-592.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of His-tagged enzyme in Escherichia coli strain JM109, coexpression with molecular chaperones GroES and GroEL Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum membrane
-
Homo sapiens 5789
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
17alpha-hydroxyprogesterone + [reduced NADPH-hemoprotein reductase] + O2 Homo sapiens
-
11-deoxycortisol + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
progesterone + [reduced NADPH-hemoprotein reductase] + O2 Homo sapiens
-
11-deoxycorticosterone + [oxidized NADPH-hemoprotein reductase] + H2O
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain JM109 by nickel affinity chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
17alpha-hydroxyprogesterone + [reduced NADPH-hemoprotein reductase] + O2
-
Homo sapiens 11-deoxycortisol + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
progesterone + [reduced NADPH-hemoprotein reductase] + O2
-
Homo sapiens 11-deoxycorticosterone + [oxidized NADPH-hemoprotein reductase] + H2O
-
?

Synonyms

Synonyms Comment Organism
CYP21
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
assay at Homo sapiens

General Information

General Information Comment Organism
physiological function CYP21 catalyses reactions of progesterone and 17alpha-hydroxyprogesterone conversion to 11-deoxycorticosterone and 11-deoxycortisol, respectively, providing an important step in aldosterone and cortisol biosynthesis. Direct molecular interactions, with electrostatic interactions playing a crucial role, between steroidogenic enzymes CYP17, EC 1.14.99.9, and CYP21 that are localized in endoplasmic reticulum membranes of adrenal cortex and involved in biosynthesis of corticosteroid hormones. The interaction in vitro reduces the catalytic activities of both enzymes at high ionic strength, i.e. 300 mM NaCl, while it increases activity at low ionic strength, i.e. 100 mM NaCl, overview Homo sapiens