Cloned (Comment) | Organism |
---|---|
mutant enzymes are expressed in Saccharomyces cerevisiae strain W303B | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
A265V | the mutant enzyme activity is similar to wild type | Homo sapiens |
D322G | the mutation impacts significantly on enzyme function and exerts activity compatible with non-classical congenital adrenal hyperplasia, has about 27% activity for the conversion of progesterone to 11-deoxycorticosterone and 18% activity for the conversion of 17alpha-hydroxyprogesterone to 11-deoxycortisol compared to wild type activity | Homo sapiens |
W302S | the mutation impacts significantly on enzyme function and exerts activity compatible with simple virilizing congenital adrenal hyperplasia, has residual enzyme activity of about 3% compared to wild type activity for both, the conversion of progesterone to 11-deoxycorticosterone and the conversion of 17alpha-hydroxyprogesterone to 11-deoxycortisol | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00072 | - |
progesterone | mutant enzyme D322G, in 50 mM potassium phosphate buffer (pH 7.4), at 37°C | Homo sapiens | |
0.00072 | - |
progesterone | wild type enzyme, in 50 mM potassium phosphate buffer (pH 7.4), at 37°C | Homo sapiens | |
0.00079 | - |
progesterone | mutant enzyme A265V, in 50 mM potassium phosphate buffer (pH 7.4), at 37°C | Homo sapiens | |
0.00135 | - |
17alpha-hydroxyprogesterone | mutant enzyme D322G, in 50 mM potassium phosphate buffer (pH 7.4), at 37°C | Homo sapiens | |
0.00138 | - |
17alpha-hydroxyprogesterone | wild type enzyme, in 50 mM potassium phosphate buffer (pH 7.4), at 37°C | Homo sapiens | |
0.00181 | - |
17alpha-hydroxyprogesterone | mutant enzyme A265V, in 50 mM potassium phosphate buffer (pH 7.4), at 37°C | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
17alpha-hydroxyprogesterone + NADPH + H+ + O2 | - |
Homo sapiens | 11-deoxycortisol + NADP+ + H2O | - |
? | |
progesterone + NADPH + H+ + O2 | - |
Homo sapiens | deoxycorticosterone + NADP+ + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CYP21A2 | - |
Homo sapiens |
Steroid 21-hydroxylase | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Homo sapiens |