BRENDA - Enzyme Database show
show all sequences of 1.14.14.158

Expression and functional analysis of Citrus carotene hydroxylases unravelling the xanthophyll biosynthesis in citrus fruits

Ma, G.; Zhang, L.; Yungyuen, W.; Tsukamoto, I.; Iijima, N.; Oikawa, M.; Yamawaki, K.; Yahata, M.; Kato, M.; BMC Plant Biol. 16, 148 (2016)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene CitCYP97C, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis. When CitHYb and CitCYP97C are coexpressed in Escherichia coli strain BL21(DE3) the monohydroxylated zeinoxanthin, which is produced by CitHYb, is further converted to lutein by CitCYP97C, when CitCYP97A and CitCYP97C are coexpressed or CitCYP97B and CitCYP97C are coexpressed, no hydroxylated carotene is detected in the alpha-carotene- and beta-carotene-accumulating Escherichia coli strain BL21(DE3) cells
Citrus unshiu
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
chloroplast
as most carotenoids are synthesized and stored in plastids, the location of CitCYP97C, together with the other enzymes involved, i.e. CitHYb, CitCYP97A, and CitCYP97B, within plastid allows them to catalyze the reaction of carotene hydroxylation
Citrus unshiu
9507
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
zeinoxanthin + [reduced NADPH-hemoprotein reductase] + O2
Citrus unshiu
-
lutein + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Citrus unshiu
A0A1L7NT18
-
-
Purification (Commentary)
Commentary
Organism
partially, isolation of CitCYP79C from fruits
Citrus unshiu
Source Tissue
Source Tissue
Commentary
Organism
Textmining
fruit
in flavedo and juice sacs, expression of CitCYP97C increases with two peaks in September and November, respectively
Citrus unshiu
-
additional information
lutein content in the juice sacs during the ripening process is high during November and December, and in the flavedo it is highest in August, while lowest in December, overview
Citrus unshiu
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
zeinoxanthin + [reduced NADPH-hemoprotein reductase] + O2
-
744606
Citrus unshiu
lutein + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
zeinoxanthin + [reduced NADPH-hemoprotein reductase] + O2
recombinant enzyme
744606
Citrus unshiu
lutein + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 61600, about, sequence calculation of CitCYP97C
Citrus unshiu
Cofactor
Cofactor
Commentary
Organism
Structure
cytochrome P450
-
Citrus unshiu
heme
-
Citrus unshiu
Cloned(Commentary) (protein specific)
Commentary
Organism
gene CitCYP97C, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis. When CitHYb and CitCYP97C are coexpressed in Escherichia coli strain BL21(DE3) the monohydroxylated zeinoxanthin, which is produced by CitHYb, is further converted to lutein by CitCYP97C, when CitCYP97A and CitCYP97C are coexpressed or CitCYP97B and CitCYP97C are coexpressed, no hydroxylated carotene is detected in the alpha-carotene- and beta-carotene-accumulating Escherichia coli strain BL21(DE3) cells
Citrus unshiu
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cytochrome P450
-
Citrus unshiu
heme
-
Citrus unshiu
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
chloroplast
as most carotenoids are synthesized and stored in plastids, the location of CitCYP97C, together with the other enzymes involved, i.e. CitHYb, CitCYP97A, and CitCYP97B, within plastid allows them to catalyze the reaction of carotene hydroxylation
Citrus unshiu
9507
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
zeinoxanthin + [reduced NADPH-hemoprotein reductase] + O2
Citrus unshiu
-
lutein + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
partially, isolation of CitCYP79C from fruits
Citrus unshiu
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
fruit
in flavedo and juice sacs, expression of CitCYP97C increases with two peaks in September and November, respectively
Citrus unshiu
-
additional information
lutein content in the juice sacs during the ripening process is high during November and December, and in the flavedo it is highest in August, while lowest in December, overview
Citrus unshiu
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
zeinoxanthin + [reduced NADPH-hemoprotein reductase] + O2
-
744606
Citrus unshiu
lutein + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
zeinoxanthin + [reduced NADPH-hemoprotein reductase] + O2
recombinant enzyme
744606
Citrus unshiu
lutein + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 61600, about, sequence calculation of CitCYP97C
Citrus unshiu
General Information
General Information
Commentary
Organism
metabolism
the enzyme is involved in the carotenoid biosynthesis pathway
Citrus unshiu
physiological function
of the four citrus carotene hydroxylases presented in four distinct clusters, CitCYP97C is the one responsible for epsilon-ring hydroxylation in Citrus unshiu, while CitCYP97A and CitCYP97B hydroxylate the alpha- and beta-rings of alpha-carotene, respectively, roles of four carotene hydroxylase genes (CitHYb, CitCYP97A, CitCYP97B, and CitCYP97C) in regulating xanthophylls biosynthesis. Zeaxanthin increases significantly during the ripening process in Citrus fruits, contents of alpha-carotene and lutein increase gradually in the juice sacs during the ripening process, the content of beta-cryptoxanthin, the major carotenoid in Satsuma mandarin, increases significantly during the ripening process in December, overview
Citrus unshiu
General Information (protein specific)
General Information
Commentary
Organism
metabolism
the enzyme is involved in the carotenoid biosynthesis pathway
Citrus unshiu
physiological function
of the four citrus carotene hydroxylases presented in four distinct clusters, CitCYP97C is the one responsible for epsilon-ring hydroxylation in Citrus unshiu, while CitCYP97A and CitCYP97B hydroxylate the alpha- and beta-rings of alpha-carotene, respectively, roles of four carotene hydroxylase genes (CitHYb, CitCYP97A, CitCYP97B, and CitCYP97C) in regulating xanthophylls biosynthesis. Zeaxanthin increases significantly during the ripening process in Citrus fruits, contents of alpha-carotene and lutein increase gradually in the juice sacs during the ripening process, the content of beta-cryptoxanthin, the major carotenoid in Satsuma mandarin, increases significantly during the ripening process in December, overview
Citrus unshiu
Other publictions for EC 1.14.14.158
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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Solanum lycopersicum
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1
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Arabidopsis thaliana
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1627-1639
1996
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