BRENDA - Enzyme Database show
show all sequences of 1.14.14.158

Overexpression of a tomato carotenoid epsilon-hydroxylase gene alleviates sensitivity to chilling stress in transgenic tobacco

Zhou, B.; Deng, Y.S.; Kong, F.Y.; Li, B.; Meng, Q.W.; Plant Physiol. Biochem. 70, 235-245 (2013)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene LeLUT1, DNA and amino acid sequence determination and analysis, quantitative RT-PCR expression analysis, expression of gene LeLUT1-GFP fusion protein in chloroplasts of Arabidopsis thaliana mesophyll protoplasts, quantitative real-time PCR expression analysis
Solanum lycopersicum
Engineering
Amino acid exchange
Commentary
Organism
additional information
construction of transgenic Nicotiana tabacum plants overexpressing LeLUT1, the transgenic plants have a higher lutein content, which is decreased in cold condition. Under chilling stress, the non-photochemical quenching values are higher in the transgenic plants than in the wild-type plants. Compared with the wild-type plants, the transgenic plants show lower levels of hydrogen peroxide, superoxide radical, relative electrical conductivity, and malondialdehyde content, and relatively higher values of maximal photochemical efficiency of photosystem II, oxidizable P700 of PSI, and net photosynthetic rate. The transgenic seedlings are less suppressed in growth and lose less cotyledon chlorophyll than the wild-type seedlings
Solanum lycopersicum
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
chloroplast
-
Solanum lycopersicum
9507
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
zeinoxanthin + [reduced NADPH-hemoprotein reductase] + O2
Solanum lycopersicum
-
lutein + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Solanum lycopersicum
BT012891
mRNA; cv. Zhongshu 4, gene LUT1
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
leaf
expression of LeLUT1 is the highest in the leaves in tomato
Solanum lycopersicum
-
additional information
expression pattern in various tomato organs, quantitative expression analysis, overview
Solanum lycopersicum
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
zeinoxanthin + [reduced NADPH-hemoprotein reductase] + O2
-
728502
Solanum lycopersicum
lutein + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Cloned(Commentary) (protein specific)
Commentary
Organism
gene LeLUT1, DNA and amino acid sequence determination and analysis, quantitative RT-PCR expression analysis, expression of gene LeLUT1-GFP fusion protein in chloroplasts of Arabidopsis thaliana mesophyll protoplasts, quantitative real-time PCR expression analysis
Solanum lycopersicum
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
construction of transgenic Nicotiana tabacum plants overexpressing LeLUT1, the transgenic plants have a higher lutein content, which is decreased in cold condition. Under chilling stress, the non-photochemical quenching values are higher in the transgenic plants than in the wild-type plants. Compared with the wild-type plants, the transgenic plants show lower levels of hydrogen peroxide, superoxide radical, relative electrical conductivity, and malondialdehyde content, and relatively higher values of maximal photochemical efficiency of photosystem II, oxidizable P700 of PSI, and net photosynthetic rate. The transgenic seedlings are less suppressed in growth and lose less cotyledon chlorophyll than the wild-type seedlings
Solanum lycopersicum
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
chloroplast
-
Solanum lycopersicum
9507
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
zeinoxanthin + [reduced NADPH-hemoprotein reductase] + O2
Solanum lycopersicum
-
lutein + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
leaf
expression of LeLUT1 is the highest in the leaves in tomato
Solanum lycopersicum
-
additional information
expression pattern in various tomato organs, quantitative expression analysis, overview
Solanum lycopersicum
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
zeinoxanthin + [reduced NADPH-hemoprotein reductase] + O2
-
728502
Solanum lycopersicum
lutein + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Expression
Organism
Commentary
Expression
Solanum lycopersicum
expression of LeLUT1 is downregulated by various abiotic stresses in tomato
down
General Information
General Information
Commentary
Organism
metabolism
the enzyme is involved in the biosynthetic pathway of lutein in plants, overview
Solanum lycopersicum
physiological function
the overexpression of gene LeLUT1 has a key function in alleviating photoinhibition and photooxidation, and decreases the sensitivity of photosynthesis to chilling stress
Solanum lycopersicum
General Information (protein specific)
General Information
Commentary
Organism
metabolism
the enzyme is involved in the biosynthetic pathway of lutein in plants, overview
Solanum lycopersicum
physiological function
the overexpression of gene LeLUT1 has a key function in alleviating photoinhibition and photooxidation, and decreases the sensitivity of photosynthesis to chilling stress
Solanum lycopersicum
Expression (protein specific)
Organism
Commentary
Expression
Solanum lycopersicum
expression of LeLUT1 is downregulated by various abiotic stresses in tomato
down
Other publictions for EC 1.14.14.158
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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Overexpression of a tomato car ...
Solanum lycopersicum
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70
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2013
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6
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6
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716454
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Over-expression of Arabidopsis ...
Arabidopsis thaliana
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Arabidopsis thaliana
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101
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716526
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Arabidopsis thaliana
Plant Cell
8
1627-1639
1996
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