Application | Comment | Organism |
---|---|---|
drug development | non-conservative Phe497 and Phe220 of CYP51 may be key residues for design of potent specific fungicides for CYP51 | Pyricularia grisea |
Cloned (Comment) | Organism |
---|---|
full-length and truncated CYP51 fragments cloned into vector pET30 and expressed in Escherichia coli strain BL21 (DE3) pLysS | Pyricularia grisea |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diniconazole | is the most sensitive fungicide for CYP51, has a stronger affinity for CYP51 than the other fungicides | Pyricularia grisea | |
additional information | affinity of the azoles for CYP51 is positively correlated with their hydrophobicity. Amino acid residues Tyr112, Phe120, Phe220, His308 and Phe497 of CYP51, forming a large hydrophobic cavity, are the key residues interacting with azole fungicides | Pyricularia grisea | |
tebuconazole | - |
Pyricularia grisea | |
triadimefon | is the less sensitive fungicide for CYP51, affinity for CYP51 is the weakest | Pyricularia grisea | |
triadimenol | hydrophobicity of triadimenol is reduced by the shortening of the carbon chain and the addition of a polar oxygen atom in comparison with tebuconazole, which may be the cause of the decrease in the affinity of triadimenol compared with tebuconazole | Pyricularia grisea |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | - |
Pyricularia grisea |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
51000 | - |
sequence analysis, truncated CYP51 | Pyricularia grisea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyricularia grisea | - |
- |
- |
Synonyms | Comment | Organism |
---|---|---|
CYP51 | - |
Pyricularia grisea |
sterol 14alpha-demethylase | - |
Pyricularia grisea |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome P450 | - |
Pyricularia grisea |
General Information | Comment | Organism |
---|---|---|
physiological function | truncated CYP51 is successfully expressed in Escherichia coli, whereas the full-length CYP51 is not | Pyricularia grisea |