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Literature summary for 1.14.14.154 extracted from

  • Warrilow, A.; Ugochukwu, C.; Lamb, D.; Kelly, D.; Kelly, S.
    Expression and characterization of CYP51, the ancient sterol 14-demethylase activity for cytochromes P450 (CYP), in the white-rot fungus Phanerochaete chrysosporium (2008), Lipids, 43, 1143-1153.
    View publication on PubMed
Search for more literature for 1.14.14.154

Cloned(Commentary)

Cloned (Comment) Organism
cloned from a cDNA library into pGEMT-Easy vector. The pCWori+ vector transformed into Escherichia coli strain DH5alpha. Expressed in yeast YUG73 from pYES2.1 vector Phanerodontia chrysosporium

Inhibitors

Inhibitors Comment Organism Structure
(2R,4S)-ketoconazole
-
 Phanerodontia chrysosporium
clotrimazole
-
 Phanerodontia chrysosporium
fluconazole
-
 Phanerodontia chrysosporium
itraconazole
-
 Phanerodontia chrysosporium
voriconazole
-
 Phanerodontia chrysosporium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
62000
-
 sequence analysis Phanerodontia chrysosporium
63000
-
 x * 63000, SDS-PAGE Phanerodontia chrysosporium

Organism

Organism UniProt Comment Textmining
Phanerodontia chrysosporium Q68HC3
-
-

Purification (Commentary)

Purification (Comment) Organism
by Ni2+-NTA affinity chromatography, more than 90% pure Phanerodontia chrysosporium

Storage Stability

Storage Stability Organism
-80°C Phanerodontia chrysosporium

Subunits

Subunits Comment Organism
? x * 63000, SDS-PAGE Phanerodontia chrysosporium

Synonyms

Synonyms Comment Organism
CYP51
-
 Phanerodontia chrysosporium
sterol 14alpha-demethylase
-
 Phanerodontia chrysosporium

Cofactor

Cofactor Comment Organism Structure
cytochrome P450
-
 Phanerodontia chrysosporium

pI Value

Organism Comment pI Value Maximum pI Value
Phanerodontia chrysosporium sequence analysis
-
 6.27

General Information

General Information Comment Organism
physiological function complementation of a conditional knock-down mutant of yeast CYP51. The CYP51 protein exhibits high affinity for azole antifungal compounds Phanerodontia chrysosporium