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Literature summary for 1.14.14.154 extracted from

  • Kitahama, Y.; Nakamura, M.; Yoshida, Y.; Aoyama, Y.
    The construction and characterization of self-sufficient lanosterol 14-demethylase fusion proteins consisting of yeast CYP51 and its reductase (2009), Biol. Pharm. Bull., 32, 558-563.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
CYP51 cDNA cloned with pBluescript KS-. Self sufficient lanosterol 14-demethylase fusion proteins by using yeast CYP51 and P450 reductase, expressed in Escherichia coli Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
ketoconazole
-
Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.004
-
lanosterol fusion protein CYP51 (F1) consisting of the ORF of modified CYP51, a linker region containing the amino acid sequence S-T, and DELTA41 P450 reductase Saccharomyces cerevisiae
0.0044
-
lanosterol fusion protein CYP51 (F2) consisting of the ORF of modified CYP51, a linker region containing the amino acid sequence S-T-E-Q-S-A-K-K-V-R-K-K-A, and DELTA55 P450 reductase Saccharomyces cerevisiae
0.005
-
lanosterol ORF of modified CYP51 Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae P10614
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
lanosterol + NADPH + O2 artificial fused enzymes have comparable activity to the reconstituted system. Reduction of CYP51 both in the fused enzyme and the reconstituted system is biphasic and consisted of an initial fast phase followed by a slow phase Saccharomyces cerevisiae 4,4-dimethylcholesta-8,14,24-trien-3-ol + NADP+ + H2O
-
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Synonyms

Synonyms Comment Organism
CYP51
-
Saccharomyces cerevisiae
lanosterol 14-demethylase
-
Saccharomyces cerevisiae