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Literature summary for 1.14.14.154 extracted from

  • Parker, J.E.; Merkamm, M.; Manning, N.J.; Pompon, D.; Kelly, S.L.; Kelly, D.E.
    Differential azole antifungal efficacies contrasted using a Saccharomyces cerevisiae strain humanized for sterol 14 alpha-demethylase at the homologous locus (2008), Antimicrob. Agents Chemother., 52, 3597-3603.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.14.154

Application

Application Comment Organism
drug development the humanized yeast strain provides a useful tool for initial specificity testing for new drugs targeting CYP51 and clearly differentiates azole antifungals in a side-by-side comparison Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Saccharomyces cerevisiae BY4741 under the control of the yeast CYP51 promoter Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
clotrimazole
-
 Homo sapiens
clotrimazole
-
 Saccharomyces cerevisiae
econazole
-
 Homo sapiens
econazole
-
 Saccharomyces cerevisiae
fluconazole
-
 Homo sapiens
fluconazole
-
 Saccharomyces cerevisiae
itraconazole
-
 Homo sapiens
itraconazole
-
 Saccharomyces cerevisiae
ketoconazole
-
 Homo sapiens
ketoconazole
-
 Saccharomyces cerevisiae
miconazole
-
 Homo sapiens
miconazole
-
 Saccharomyces cerevisiae
additional information humanized yeast strain as compared to the parental yeast strain shows up to 1000fold reduced susceptibility to the orally active azole drugs and reduced effectiveness for the topical drugs Homo sapiens
additional information the parental yeast strain as compared to humanized yeast strain shows up to 1000fold higher susceptibility to the orally active azole drugs Saccharomyces cerevisiae
voriconazole displays the greatest specificity Homo sapiens
voriconazole displays the greatest specificity Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Homo sapiens 16020
-
microsome
-
 Homo sapiens
-
-

Organism

Organism UniProt Comment Textmining
Homo sapiens Q16850
-
-
Saccharomyces cerevisiae
-
-
-
Saccharomyces cerevisiae BY4741
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
lanosterol + [reduced NADPH-hemoprotein reductase] + O2
-
 Saccharomyces cerevisiae ? + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
lanosterol + [reduced NADPH-hemoprotein reductase] + O2
-
 Homo sapiens ? + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
lanosterol + [reduced NADPH-hemoprotein reductase] + O2
-
 Saccharomyces cerevisiae BY4741 ? + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Synonyms

Synonyms Comment Organism
CYP51
-
 Saccharomyces cerevisiae
CYP51
-
 Homo sapiens
Erg11p
-
 Saccharomyces cerevisiae
Erg11p
-
 Homo sapiens