Application | Comment | Organism |
---|---|---|
drug development | CYP51 is a key target for fungal antibiotic therapy | Mycobacterium tuberculosis |
pharmacology | CYP51 is a key target for fungal antibiotic therapy | Mycobacterium tuberculosis |
Crystallization (Comment) | Organism |
---|---|
recombinant mutant C37L/C442A in complex with alpha-ethyl-N-4-pyridinyl-benzeneacetamide, protein is mixed with a ligand dissolved in DMSO at a 100 mM concentration to obtain a final protein concentration of 0.2 mM and a final ligand concentration of 1 to 5 mM, 15-30% PEG 4000, 2-12% isopropanol, 0.1 M HEPES, pH 7.5, X-ray diffraction at 1.53 A resolution | Mycobacterium tuberculosis |
Protein Variants | Comment | Organism |
---|---|---|
C37L/C442A | site-directed mutagenesis, structure determination | Mycobacterium tuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-(benzo[d]-2,1,3-thiadiazole-4-sulfonyl)-2-amino-2-phenyl-N-(pyridinyl-4)-acetamide | binding structure, overview | Mycobacterium tuberculosis | |
alpha-ethyl-N-4-pyridinyl-benzeneacetamide | binds to the non-heme iron, binding structure involving residues H259 and Y76, overview | Mycobacterium tuberculosis | |
additional information | specific inhibitor screening | Mycobacterium tuberculosis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Mycobacterium tuberculosis | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | a cytochrome P450 enzyme, non-heme iron | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Mycobacterium tuberculosis | CYP51 is a major checkpoint in membrane sterol biosynthesis, is a key target for fungal antibiotic therapy | ? | - |
? | |
additional information | Mycobacterium tuberculosis H37Rv | CYP51 is a major checkpoint in membrane sterol biosynthesis, is a key target for fungal antibiotic therapy | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P9WPP9 | - |
- |
Mycobacterium tuberculosis H37Rv | P9WPP9 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | CYP51 is a major checkpoint in membrane sterol biosynthesis, is a key target for fungal antibiotic therapy | Mycobacterium tuberculosis | ? | - |
? | |
additional information | CYP51 is a major checkpoint in membrane sterol biosynthesis, is a key target for fungal antibiotic therapy | Mycobacterium tuberculosis H37Rv | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CYP51 | - |
Mycobacterium tuberculosis |
sterol 14alpha-demethylase | - |
Mycobacterium tuberculosis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome P450 | a cytochrome P450 enzyme | Mycobacterium tuberculosis |