Any feedback?
Literature summary for 1.14.14.154 extracted from
- Sterol 14alpha-demethylase, an abundant and essential mixed-function oxidase (2005), Biochem. Biophys. Res. Commun., 338, 418-422.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Iron | the enzyme is a fusion protein with a 3Fe-4S ferredoxin | Methylococcus capsulatus |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Mycobacterium tuberculosis |
| the genes are located on chromosomes 3, 7, and 13, the gene on chromosome 7 is the functional one while those on chromosomes 3 and 13 are processed pseudogenes which arise from reverse transcription of mRNA in germ cells and illegitimate recombination into the genome, CYP51 responds to cholesterol feedback regulation, being upregulated in sterol limiting conditions and downregulated in cholesterol rich conditions, regulation involves the sterol regulatory elements SRE in the promoter of the gene which bind sterol regulatory element binding protein, SREBP, the CYP51 promoter also contains a cAMP regulatory element, CRE, binding cAMP regulatory proteins CREB/CREM | Homo sapiens |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| purified recombinant enzyme, crystal structure determination and analysis, overview | Mycobacterium tuberculosis |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| endoplasmic reticulum | - |
Homo sapiens | 5783 | - |
| membrane | - |
Homo sapiens | 16020 | - |
| membrane | - |
Trypanosoma brucei | 16020 | - |
| membrane | - |
Methylococcus capsulatus | 16020 | - |
| soluble | - |
Mycobacterium tuberculosis | - |
- |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 24,25-dihydrolanosterol + [reduced NADPH-hemoprotein reductase] + O2 | Mycobacterium tuberculosis | - |
? | - |
? | |
| 24,25-dihydrolanosterol + [reduced NADPH-hemoprotein reductase] + O2 | Acanthamoeba polyphaga | - |
? | - |
? | |
| lanosterol + [reduced NADPH-hemoprotein reductase] + O2 | Mycobacterium tuberculosis | - |
? | - |
? | |
| lanosterol + [reduced NADPH-hemoprotein reductase] + O2 | Acanthamoeba polyphaga | - |
? | - |
? | |
| lanosterol + [reduced NADPH-hemoprotein reductase] + O2 | Homo sapiens | CYP51 responds to cholesterol feedback regulation, being upregulated in sterol limiting conditions and downregulated in cholesterol rich conditions, regulation involves the sterol regulatory elements SRE in the promoter of the gene which bind sterol regulatory element binding protein, SREBP, the CYP51 promoter also contains a cAMP regulatory element, CRE, binding cAMP regulatory proteins CREB/CREM, overview | ? | - |
? | |
| additional information | Methylococcus capsulatus | CYP51 participates in biosynthesis of cholesterol | ? | - |
? | |
| additional information | Homo sapiens | CYP51 plays a key role in fertilization by producing intermediates that could serve as ligands for nuclear receptors | ? | - |
? | |
| norlanosterol + [reduced NADPH-hemoprotein reductase] + O2 | Mycobacterium tuberculosis | - |
? | - |
? | |
| norlanosterol + [reduced NADPH-hemoprotein reductase] + O2 | Acanthamoeba polyphaga | - |
? | - |
? | |
| obtusifoliol + [reduced NADPH-hemoprotein reductase] + O2 | Mycobacterium tuberculosis | - |
4alpha-methyl-5alpha-ergost-8,14,24(28)-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| obtusifoliol + [reduced NADPH-hemoprotein reductase] + O2 | Acanthamoeba polyphaga | - |
4alpha-methyl-5alpha-ergost-8,14,24(28)-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acanthamoeba polyphaga | - |
- |
- |
| Homo sapiens | - |
- |
- |
| Methylococcus capsulatus | - |
- |
- |
| Mycobacterium tuberculosis | - |
- |
- |
| Trypanosoma brucei | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| Leydig cell | - |
Homo sapiens | - |
| liver | - |
Homo sapiens | - |
| ovary | - |
Homo sapiens | - |
| spermatid | acrosomal membrane of elongated spermatids | Homo sapiens | - |
| spermatozoon | acrosomal membrane of mature sperm | Homo sapiens | - |
| testis | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 24,25-dihydrolanosterol + [reduced NADPH-hemoprotein reductase] + O2 | - |
Mycobacterium tuberculosis | ? | - |
? | |
| 24,25-dihydrolanosterol + [reduced NADPH-hemoprotein reductase] + O2 | - |
Acanthamoeba polyphaga | ? | - |
? | |
| lanosterol + [reduced NADPH-hemoprotein reductase] + O2 | - |
Homo sapiens | ? | - |
? | |
| lanosterol + [reduced NADPH-hemoprotein reductase] + O2 | - |
Mycobacterium tuberculosis | ? | - |
? | |
| lanosterol + [reduced NADPH-hemoprotein reductase] + O2 | - |
Acanthamoeba polyphaga | ? | - |
? | |
| lanosterol + [reduced NADPH-hemoprotein reductase] + O2 | CYP51 responds to cholesterol feedback regulation, being upregulated in sterol limiting conditions and downregulated in cholesterol rich conditions, regulation involves the sterol regulatory elements SRE in the promoter of the gene which bind sterol regulatory element binding protein, SREBP, the CYP51 promoter also contains a cAMP regulatory element, CRE, binding cAMP regulatory proteins CREB/CREM, overview | Homo sapiens | ? | - |
? | |
| additional information | CYP51 participates in biosynthesis of cholesterol | Methylococcus capsulatus | ? | - |
? | |
| additional information | CYP51 plays a key role in fertilization by producing intermediates that could serve as ligands for nuclear receptors | Homo sapiens | ? | - |
? | |
| norlanosterol + [reduced NADPH-hemoprotein reductase] + O2 | - |
Mycobacterium tuberculosis | ? | - |
? | |
| norlanosterol + [reduced NADPH-hemoprotein reductase] + O2 | - |
Acanthamoeba polyphaga | ? | - |
? | |
| obtusifoliol + [reduced NADPH-hemoprotein reductase] + O2 | - |
Mycobacterium tuberculosis | 4alpha-methyl-5alpha-ergost-8,14,24(28)-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| obtusifoliol + [reduced NADPH-hemoprotein reductase] + O2 | - |
Acanthamoeba polyphaga | 4alpha-methyl-5alpha-ergost-8,14,24(28)-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CYP51 | - |
Homo sapiens |
| CYP51 | - |
Trypanosoma brucei |
| CYP51 | - |
Mycobacterium tuberculosis |
| CYP51 | - |
Acanthamoeba polyphaga |
| sterol 14alpha-demethylase | - |
Homo sapiens |
| sterol 14alpha-demethylase | - |
Trypanosoma brucei |
| sterol 14alpha-demethylase | - |
Mycobacterium tuberculosis |
| sterol 14alpha-demethylase | - |
Methylococcus capsulatus |
| sterol 14alpha-demethylase | - |
Acanthamoeba polyphaga |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| Ferredoxin | enzyme-bound, the enzyme is a fusion protein with a 3Fe-4S ferredoxin | Methylococcus capsulatus |
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Release 2023.1 (January 2023)
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