Application | Comment | Organism |
---|---|---|
medicine | the enzyme is a target in treatment of the chronic lung disease caused by Mycobacterium avium | Mycobacterium avium subsp. avium |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli as GST-tagged enzyme | Mycobacterium avium subsp. avium |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
econazole | - |
Mycobacterium avium subsp. avium | |
fluconazole | - |
Mycobacterium avium subsp. avium | |
itraconazole | - |
Mycobacterium avium subsp. avium | |
ketoconazole | - |
Mycobacterium avium subsp. avium | |
voriconazole | - |
Mycobacterium avium subsp. avium |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | - |
Mycobacterium avium subsp. avium |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
lanosterol + [reduced NADPH-hemoprotein reductase] + O2 | Mycobacterium avium subsp. avium | - |
? | - |
? | |
obtusifoliol + [reduced NADPH-hemoprotein reductase] + O2 | Mycobacterium avium subsp. avium | preferred substrate | 4alpha-methyl-5alpha-ergost-8,14,24(28)-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium avium subsp. avium | Q3HTM1 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant GST-tagged enzyme from Escherichia coli by glutathione affinity chromatography, cleavage of the tag by thrombin, and thrombin elimination by benzamidine affinity chromatography | Mycobacterium avium subsp. avium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
lanosterol + [reduced NADPH-hemoprotein reductase] + O2 | - |
Mycobacterium avium subsp. avium | ? | - |
? | |
obtusifoliol + [reduced NADPH-hemoprotein reductase] + O2 | preferred substrate | Mycobacterium avium subsp. avium | 4alpha-methyl-5alpha-ergost-8,14,24(28)-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CYP51 | - |
Mycobacterium avium subsp. avium |
lanosterol 14alpha-demethylase | - |
Mycobacterium avium subsp. avium |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Mycobacterium avium subsp. avium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Mycobacterium avium subsp. avium |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
Ferredoxin | - |
Mycobacterium avium subsp. avium |