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Literature summary for 1.14.14.154 extracted from
- Properties and structural requirements for substrate specificity of cytochrome P-450-dependent obtusifoliol 14alpha-demethylase from maize (Zea mays) seedlings (1991), Biochem. J., 277, 483-492.
Application
| Application | Comment | Organism |
|---|---|---|
| agriculture | target enzyme for azole antifungal agents. These specific inhibitors are of great importance as plant growth regulators, fungicides and herbicides in the agricultural and medical fields | Zea mays |
| agriculture | target of important agrochemicals such as fungicides, plant growth regulators and herbicides | Zea mays |
| medicine | target enzyme for azole antifungal agents. These specific inhibitors are of great importance as plant growth regulators, fungicides and herbicides in the agricultural and medical fields | Zea mays |
| pharmacology | target enzyme for azole antifungal agents. These specific inhibitors are of great importance as plant growth regulators, fungicides and herbicides in the agricultural and medical fields | Zea mays |
General Stability
| General Stability | Organism |
|---|---|
| plant demethylase is remarkably stable | Zea mays |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetic parameters | Zea mays | |
| 0.116 | - |
(3beta,4alpha,5alpha)-4,14-dimethylcholest-8-en-3-ol | - |
Zea mays |  |
| 0.16 | - |
obtusifoliol | - |
Zea mays | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| endoplasmic reticulum | - |
Zea mays | 5783 | - |
| endoplasmic reticulum | light membrane fraction of endoplasmic reticulum, not in plasma membrane | Zea mays | 5783 | - |
| membrane | membrane-bound | Zea mays | 16020 | - |
| microsome | microsomal-bound | Zea mays | - |
- |
| additional information | not in plasma membrane | Zea mays | - |
- |
| additional information | subcellular localization, distribution | Zea mays | - |
- |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Zea mays | enzyme of sterol biosynthetic pathway | ? | - |
? | |
| additional information | Zea mays | enzyme of plant sterol, phytosterol, biosynthesis | ? | - |
? | |
| obtusifoliol + [reduced NADPH-hemoprotein reductase] + O2 | Zea mays | 4alpha,14alpha-dimethyl-24-methylene-5alpha-cholesta-8-en-3beta-ol | 4alpha-methyl-5alpha-ergosta-8,14,24(28)-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| obtusifoliol + [reduced NADPH-hemoprotein reductase] + O2 | Zea mays | 4alpha,14alpha-dimethyl-5alpha-ergosta-8,24(28)-dien-3beta-ol | 4alpha-methyl-5alpha-ergosta-8,14,24(28)-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Zea mays | - |
maize | - |
| Zea mays | - |
LG11 | - |
| Zea mays LG11 | - |
LG11 | - |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| a 14alpha-methylsteroid + 3 [reduced NADPH-hemoprotein reductase] + 3 O2 = a DELTA14-steroid + formate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O | mechanism | Zea mays |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seedling | - |
Zea mays | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (3b,4a,5a)-4,14-dimethylcholest-8-en-3-ol + [reduced NADPH-hemoprotein reductase] + O2 | good substrate, 67% of activity compared to obtusifoliol | Zea mays | ? + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| (3beta,4alpha,5alpha)-4,14-dimethylcholest-8-en-3-ol + [reduced NADPH-hemoprotein reductase] + O2 | - |
Zea mays | ? | - |
r | |
| 14alpha-methyl-24,28-dihydrofecosterol + [reduced NADPH-hemoprotein reductase] + O2 | 50% of activity compared to obtusifoliol | Zea mays | ? + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 24,28-dihydroobtusifoliol + [reduced NADPH-hemoprotein reductase] + O2 | good substrate, 75% of activity to obtusifoliol | Zea mays | 4alpha-methyl-5alpha-ergosta-8,14-dien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 24,28-dihydroobtusifoliol + [reduced NADPH-hemoprotein reductase] + O2 | DHO | Zea mays | 4alpha-methyl-5alpha-ergosta-8,14-dien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 24,28-dihydroobtusifoliol + [reduced NADPH-hemoprotein reductase] + O2 | 4alpha,14alpha-dimethyl-5alpha-ergosta-8-en-3beta-ol | Zea mays | 4alpha-methyl-5alpha-ergosta-8,14-dien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| additional information | substrate specificity | Zea mays | ? | - |
? | |
| additional information | no activity with 31-norlanosterol, cycloeucalenol, 4alpha,14alpha-dimethyl-5alpha-ergost-9(11)-en-3beta-ol, 4alpha,14alpha-dimethyl-5alpha-ergost-7-en-3beta-ol, 8(9),24(25)-tetrahydro-31-norlanosterol, 24-methylenelanosterol, 24,28-dihydro-4beta-methyl-30-norobtusifoliol, 24,25-dihydrolanosterol, lanosterol, obtusifoliyl-3beta-methoxy, obtusifoliyl-3beta-acetoxy, obtusifoliyl-3beta-amino | Zea mays | ? | - |
? | |
| additional information | biosynthetic enzyme with very narrow substrate specificity | Zea mays | ? | - |
? | |
| additional information | P-450OBT 14DM has probably a specific apolar binding site for the side chain. DELTA8-double bond is absolute required for substrate demethylation and the 3-hydroxy group plays a critical role in enzyme-substrate interaction | Zea mays | ? | - |
? | |
| additional information | enzyme with high degree of substrate and product specificity | Zea mays | ? | - |
? | |
| additional information | plant sterol 14alpha-demethylase have high substrate specificity | Zea mays | ? | - |
? | |
| additional information | narrow substrate selectivity | Zea mays | ? | - |
? | |
| additional information | enzyme of sterol biosynthetic pathway | Zea mays | ? | - |
? | |
| additional information | enzyme of plant sterol, phytosterol, biosynthesis | Zea mays | ? | - |
? | |
| additional information | substrate specificity | Zea mays LG11 | ? | - |
? | |
| additional information | no activity with 31-norlanosterol, cycloeucalenol, 4alpha,14alpha-dimethyl-5alpha-ergost-9(11)-en-3beta-ol, 4alpha,14alpha-dimethyl-5alpha-ergost-7-en-3beta-ol, 8(9),24(25)-tetrahydro-31-norlanosterol, 24-methylenelanosterol, 24,28-dihydro-4beta-methyl-30-norobtusifoliol, 24,25-dihydrolanosterol, lanosterol, obtusifoliyl-3beta-methoxy, obtusifoliyl-3beta-acetoxy, obtusifoliyl-3beta-amino | Zea mays LG11 | ? | - |
? | |
| additional information | biosynthetic enzyme with very narrow substrate specificity | Zea mays LG11 | ? | - |
? | |
| additional information | P-450OBT 14DM has probably a specific apolar binding site for the side chain. DELTA8-double bond is absolute required for substrate demethylation and the 3-hydroxy group plays a critical role in enzyme-substrate interaction | Zea mays LG11 | ? | - |
? | |
| additional information | enzyme with high degree of substrate and product specificity | Zea mays LG11 | ? | - |
? | |
| obtusifoliol + [reduced NADPH-hemoprotein reductase] + O2 | best substrate | Zea mays | 4alpha-methyl-5alpha-ergosta-8,14,24(28)-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| obtusifoliol + [reduced NADPH-hemoprotein reductase] + O2 | 4alpha,14alpha-dimethyl-24-methylene-5alpha-cholesta-8-en-3beta-ol | Zea mays | 4alpha-methyl-5alpha-ergosta-8,14,24(28)-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| obtusifoliol + [reduced NADPH-hemoprotein reductase] + O2 | catalyzes 14alpha-demethylation of obtusifoliol | Zea mays | 4alpha-methyl-5alpha-ergosta-8,14,24(28)-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| obtusifoliol + [reduced NADPH-hemoprotein reductase] + O2 | 4alpha,14alpha-dimethyl-5alpha-ergosta-8,24(28)-dien-3beta-ol | Zea mays | 4alpha-methyl-5alpha-ergosta-8,14,24(28)-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Zea mays |
| 30 | - |
aerobic conditions | Zea mays |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 8.5 | - |
Zea mays |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 9.5 | - |
Zea mays |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| cytochrome P450 | - |
Zea mays | |
| heme | heme-thiolate enzyme | Zea mays | |
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