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Literature summary for 1.14.14.154 extracted from

  • Aoyama, Y.; Horiuchi, T.; Yoshida, Y.
    Lanosterol 14-demethylase activity expressed in rat brain microsomes (1996), J. Biochem., 120, 982-986.
    View publication on PubMed
Search for more literature for 1.14.14.154

Activating Compound

Activating Compound Comment Organism Structure
cytosolic carrier required for maximum activity Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
CO high partial pressure of CO, ratio CO:O2 of 95:5 causes 48% inhibition Rattus norvegicus
ketoconazole potent inhibitor, 0.015 mM: complete inhibition Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Rattus norvegicus 16020
-
microsome
-
 Rattus norvegicus
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
lanosterol + [reduced NADPH-hemoprotein reductase] + O2 Rattus norvegicus lanosta-8,24-dien-3beta-ol 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
lanosterol + [reduced NADPH-hemoprotein reductase] + O2 Rattus norvegicus 4,4,14alpha-trimethyl-5alpha-cholesta-8,24-dien-3beta-ol 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
additional information Rattus norvegicus brain microsomes, existence of sterol biosynthetic pathway in brain, cholesterol is synthesized de novo in brain ?
-
 ?
additional information Rattus norvegicus lanosterol 14-demethylation is situated at the root of sterol-biosynthetic branch of mevalonic acid pathway ?
-
 ?
additional information Rattus norvegicus enzyme of sterol biosynthetic pathway ?
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
 Sprague-Dawley rat
-

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Rattus norvegicus
-
liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0000084
-
 brain microsomes, substrate: lanosterol Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
lanosterol + [reduced NADPH-hemoprotein reductase] + O2 best substrate Rattus norvegicus 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
lanosterol + [reduced NADPH-hemoprotein reductase] + O2 lanosta-8,24-dien-3beta-ol Rattus norvegicus 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
lanosterol + [reduced NADPH-hemoprotein reductase] + O2 4,4,14alpha-trimethyl-5alpha-cholesta-8,24-dien-3beta-ol Rattus norvegicus 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
additional information brain microsomes, existence of sterol biosynthetic pathway in brain, cholesterol is synthesized de novo in brain Rattus norvegicus ?
-
 ?
additional information lanosterol 14-demethylation is situated at the root of sterol-biosynthetic branch of mevalonic acid pathway Rattus norvegicus ?
-
 ?
additional information enzyme of sterol biosynthetic pathway Rattus norvegicus ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Rattus norvegicus
37
-
 aerobic conditions Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
cytochrome P450
-
 Rattus norvegicus
heme heme-thiolate enzyme Rattus norvegicus