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Literature summary for 1.14.14.154 extracted from
- Role of the 8-double bond of lanosterol in the enzyme-substrate interaction of cytochrome P-45014DM (lanosterol 14alpha-demethylase) (1989), Biochim. Biophys. Acta, 1001, 196-200.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 7-lanostene-3beta,32-diol | activation | Saccharomyces cerevisiae |  |
| 7-lanostene-3beta-ol | activation | Saccharomyces cerevisiae | |
| 8-lanostene-3beta,32-diol | activation | Saccharomyces cerevisiae | |
| 8-lanostene-3beta-ol | activation | Saccharomyces cerevisiae | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics | Saccharomyces cerevisiae | |
| additional information | - |
additional information | enzyme shows higher affinity for 8-lanostene conformation, such as lanosterol and 24,25-dihydrolanosterol, than for 7-lanostene one | Saccharomyces cerevisiae | |
| additional information | - |
additional information | affinity and activity for 7-lanosten-3beta-ol is very low, no exact Km | Saccharomyces cerevisiae | |
| 0.0001 | - |
8-lanostene-3beta,32-diol | - |
Saccharomyces cerevisiae | |
| 0.003 | - |
7-lanostene-3beta,32-diol | - |
Saccharomyces cerevisiae | |
| 0.02 | - |
24,25-dihydrolanosterol | 8-lanosten-3beta-ol | Saccharomyces cerevisiae | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 24,25-dihydrolanosterol + [reduced NADPH-hemoprotein reductase] + O2 | Saccharomyces cerevisiae | 8-lanosta-3beta-ol | 4,4-dimethyl-5alpha-cholesta-8,14-dien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 24,25-dihydrolanosterol + [reduced NADPH-hemoprotein reductase] + O2 | Saccharomyces cerevisiae | DHL | 4,4-dimethyl-5alpha-cholesta-8,14-dien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 24,25-dihydrolanosterol + [reduced NADPH-hemoprotein reductase] + O2 | Saccharomyces cerevisiae | 8-lanosten-3beta-ol | 4,4-dimethyl-5alpha-cholesta-8,14-dien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 24,25-dihydrolanosterol + [reduced NADPH-hemoprotein reductase] + O2 | Saccharomyces cerevisiae | 4,4,14alpha-trimethyl-5alpha-cholesta-8-en-3beta-ol | 4,4-dimethyl-5alpha-cholesta-8,14-dien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| lanosterol + [reduced NADPH-hemoprotein reductase] + O2 | Saccharomyces cerevisiae | lanosta-8,24-dien-3beta-ol | 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| lanosterol + [reduced NADPH-hemoprotein reductase] + O2 | Saccharomyces cerevisiae | 4,4,14alpha-trimethyl-5alpha-cholesta-8,24-dien-3beta-ol | 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Saccharomyces cerevisiae |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| a 14alpha-methylsteroid + 3 [reduced NADPH-hemoprotein reductase] + 3 O2 = a DELTA14-steroid + formate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O | mechanism | Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 24,25-dihydrolanosterol + [reduced NADPH-hemoprotein reductase] + O2 | 8-lanosta-3beta-ol | Saccharomyces cerevisiae | 4,4-dimethyl-5alpha-cholesta-8,14-dien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 24,25-dihydrolanosterol + [reduced NADPH-hemoprotein reductase] + O2 | DHL | Saccharomyces cerevisiae | 4,4-dimethyl-5alpha-cholesta-8,14-dien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 24,25-dihydrolanosterol + [reduced NADPH-hemoprotein reductase] + O2 | 8-lanosten-3beta-ol | Saccharomyces cerevisiae | 4,4-dimethyl-5alpha-cholesta-8,14-dien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 24,25-dihydrolanosterol + [reduced NADPH-hemoprotein reductase] + O2 | 4,4,14alpha-trimethyl-5alpha-cholesta-8-en-3beta-ol | Saccharomyces cerevisiae | 4,4-dimethyl-5alpha-cholesta-8,14-dien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 7-lanosten-3beta-ol + [reduced NADPH-hemoprotein reductase] + O2 | very low activity | Saccharomyces cerevisiae | 4,4-dimethylcholesta-7,14-dien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 7-lanostene-3beta,32-diol + [reduced NADPH-hemoprotein reductase] + O2 | very low activity | Saccharomyces cerevisiae | 4,4-dimethylcholesta-7,14-dien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 8-lanostene-3beta,32-diol + [reduced NADPH-hemoprotein reductase] + O2 | - |
Saccharomyces cerevisiae | 4,4-dimethylcholesta-8,14-dien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| lanosterol + [reduced NADPH-hemoprotein reductase] + O2 | best substrate | Saccharomyces cerevisiae | 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| lanosterol + [reduced NADPH-hemoprotein reductase] + O2 | lanosta-8,24-dien-3beta-ol | Saccharomyces cerevisiae | 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| lanosterol + [reduced NADPH-hemoprotein reductase] + O2 | 4,4,14alpha-trimethyl-5alpha-cholesta-8,24-dien-3beta-ol | Saccharomyces cerevisiae | 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| additional information | 8-double bond of lanosterol plays an important critical role in enzyme-substrate interaction of cytochrome P-45014DM | Saccharomyces cerevisiae | ? | - |
? | |
| additional information | no activity with 6-lanostene-3beta,32-diol and lanostane-3beta,32-diol | Saccharomyces cerevisiae | ? | - |
? | |
| additional information | enzyme recognizes 8-lanostene structure and favourably interacts with 8-lanostene derivatives, can act also with substrates having 7-lanostene structure, utilizes them with lower efficiency than 8-lanostene derivatives | Saccharomyces cerevisiae | ? | - |
? | |
| additional information | narrow substrate selectivity | Saccharomyces cerevisiae | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Saccharomyces cerevisiae |
| 30 | - |
aerobic conditions | Saccharomyces cerevisiae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Saccharomyces cerevisiae |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| cytochrome P450 | - |
Saccharomyces cerevisiae | |
| heme | heme-thiolate enzyme | Saccharomyces cerevisiae | |
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