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Literature summary for 1.14.14.154 extracted from
- Cloning and expression in Escherichia coli of the obtusifoliol 14alpha-demethylase of Sorghum bicolor (L.) Moench, a cytochrome P450 orthologous to the sterol 14alpha-demethylases (CYP51) from fungi and mammals (1997), Plant J., 11, 191-201.
Application
| Application | Comment | Organism |
|---|---|---|
| agriculture | target enzyme for azole antifungal agents. These specific inhibitors are of great importance as plant growth regulators, fungicides and herbicides in the agricultural and medical fields | Saccharomyces cerevisiae |
| agriculture | target enzyme for azole antifungal agents. These specific inhibitors are of great importance as plant growth regulators, fungicides and herbicides in the agricultural and medical fields | Zea mays |
| agriculture | target enzyme for azole antifungal agents. These specific inhibitors are of great importance as plant growth regulators, fungicides and herbicides in the agricultural and medical fields | Sorghum bicolor |
| medicine | - |
Rattus norvegicus |
| medicine | target enzyme for azole antifungal agents. These specific inhibitors are of great importance as plant growth regulators, fungicides and herbicides in the agricultural and medical fields | Saccharomyces cerevisiae |
| medicine | target enzyme for azole antifungal agents. These specific inhibitors are of great importance as plant growth regulators, fungicides and herbicides in the agricultural and medical fields | Zea mays |
| medicine | target enzyme for azole antifungal agents. These specific inhibitors are of great importance as plant growth regulators, fungicides and herbicides in the agricultural and medical fields | Sorghum bicolor |
| pharmacology | - |
Rattus norvegicus |
| pharmacology | target enzyme for azole antifungal agents. These specific inhibitors are of great importance as plant growth regulators, fungicides and herbicides in the agricultural and medical fields | Saccharomyces cerevisiae |
| pharmacology | target enzyme for azole antifungal agents. These specific inhibitors are of great importance as plant growth regulators, fungicides and herbicides in the agricultural and medical fields | Zea mays |
| pharmacology | target enzyme for azole antifungal agents. These specific inhibitors are of great importance as plant growth regulators, fungicides and herbicides in the agricultural and medical fields | Sorghum bicolor |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| cDNA cloning of Sorghum CYP51and functional expression in Escherichia coli JM109 in high levels | Sorghum bicolor |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| azole antifungal agents | specific inhibitors | Rattus norvegicus | |
| azole antifungal agents | specific inhibitors | Saccharomyces cerevisiae | |
| azole antifungal agents | specific inhibitors | Sorghum bicolor |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Rattus norvegicus | 16020 | - |
| membrane | membrane-bound | Zea mays | 16020 | - |
| membrane | membrane-bound | Sorghum bicolor | 16020 | - |
| microsome | - |
Rattus norvegicus | - |
- |
| microsome | - |
Saccharomyces cerevisiae | - |
- |
| microsome | - |
Zea mays | - |
- |
| microsome | - |
Sorghum bicolor | - |
- |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 24,25-dihydrolanosterol + [reduced NADPH-hemoprotein reductase] + O2 | Rattus norvegicus | 8-lanosta-3beta-ol | 4,4-dimethyl-5alpha-cholesta-8,14-dien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |  |
| 24,25-dihydrolanosterol + [reduced NADPH-hemoprotein reductase] + O2 | Saccharomyces cerevisiae | 8-lanosta-3beta-ol | 4,4-dimethyl-5alpha-cholesta-8,14-dien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 24,25-dihydrolanosterol + [reduced NADPH-hemoprotein reductase] + O2 | Rattus norvegicus | DHL | 4,4-dimethyl-5alpha-cholesta-8,14-dien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 24,25-dihydrolanosterol + [reduced NADPH-hemoprotein reductase] + O2 | Saccharomyces cerevisiae | DHL | 4,4-dimethyl-5alpha-cholesta-8,14-dien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 24,25-dihydrolanosterol + [reduced NADPH-hemoprotein reductase] + O2 | Rattus norvegicus | 8-lanosten-3beta-ol | 4,4-dimethyl-5alpha-cholesta-8,14-dien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 24,25-dihydrolanosterol + [reduced NADPH-hemoprotein reductase] + O2 | Saccharomyces cerevisiae | 8-lanosten-3beta-ol | 4,4-dimethyl-5alpha-cholesta-8,14-dien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 24,25-dihydrolanosterol + [reduced NADPH-hemoprotein reductase] + O2 | Rattus norvegicus | 4,4,14alpha-trimethyl-5alpha-cholesta-8-en-3beta-ol | 4,4-dimethyl-5alpha-cholesta-8,14-dien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 24,25-dihydrolanosterol + [reduced NADPH-hemoprotein reductase] + O2 | Saccharomyces cerevisiae | 4,4,14alpha-trimethyl-5alpha-cholesta-8-en-3beta-ol | 4,4-dimethyl-5alpha-cholesta-8,14-dien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| eburicol + [reduced NADPH-hemoprotein reductase] + O2 | Penicillium italicum | - |
? + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| lanosterol + [reduced NADPH-hemoprotein reductase] + O2 | Homo sapiens | lanosta-8,24-dien-3beta-ol | 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| lanosterol + [reduced NADPH-hemoprotein reductase] + O2 | Rattus norvegicus | lanosta-8,24-dien-3beta-ol | 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| lanosterol + [reduced NADPH-hemoprotein reductase] + O2 | Saccharomyces cerevisiae | lanosta-8,24-dien-3beta-ol | 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| lanosterol + [reduced NADPH-hemoprotein reductase] + O2 | Homo sapiens | 4,4,14alpha-trimethyl-5alpha-cholesta-8,24-dien-3beta-ol | 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| lanosterol + [reduced NADPH-hemoprotein reductase] + O2 | Rattus norvegicus | 4,4,14alpha-trimethyl-5alpha-cholesta-8,24-dien-3beta-ol | 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| lanosterol + [reduced NADPH-hemoprotein reductase] + O2 | Saccharomyces cerevisiae | 4,4,14alpha-trimethyl-5alpha-cholesta-8,24-dien-3beta-ol | 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| additional information | Sorghum bicolor | biosynthetic enzyme with very narrow substrate specificity | ? | - |
? | |
| additional information | Sorghum bicolor | enzyme is a multifunctional cytochrome P450, which as the same active site catalyze demethylation in three consecutive NADPH- and O2-dependent hydroxylation reactions, resulting in the elimination of the methyl group as formic acid and the introduction of a double bond at the DELTA14 position | ? | - |
? | |
| additional information | Sorghum bicolor | catalyzes an essential step in sterol biosynthesis as evidenced by the absence of a 14alpha-methyl group in all known functional sterols, removal of the 14alpha-methyl group is essential | ? | - |
? | |
| additional information | Homo sapiens | enzyme of sterol biosynthetic pathway | ? | - |
? | |
| additional information | Rattus norvegicus | enzyme of sterol biosynthetic pathway | ? | - |
? | |
| additional information | Saccharomyces cerevisiae | enzyme of sterol biosynthetic pathway | ? | - |
? | |
| additional information | Zea mays | enzyme of sterol biosynthetic pathway | ? | - |
? | |
| additional information | Schizosaccharomyces pombe | enzyme of sterol biosynthetic pathway | ? | - |
? | |
| additional information | Candida albicans | enzyme of sterol biosynthetic pathway | ? | - |
? | |
| additional information | Candida tropicalis | enzyme of sterol biosynthetic pathway | ? | - |
? | |
| additional information | Sorghum bicolor | enzyme of sterol biosynthetic pathway | ? | - |
? | |
| additional information | Ustilago maydis | enzyme of sterol biosynthetic pathway | ? | - |
? | |
| additional information | Penicillium italicum | enzyme of sterol biosynthetic pathway | ? | - |
? | |
| additional information | Zea mays | enzyme of plant sterol, phytosterol, biosynthesis | ? | - |
? | |
| additional information | Sorghum bicolor | enzyme of plant sterol, phytosterol, biosynthesis | ? | - |
? | |
| obtusifoliol + [reduced NADPH-hemoprotein reductase] + O2 | Zea mays | 4alpha,14alpha-dimethyl-24-methylene-5alpha-cholesta-8-en-3beta-ol | 4alpha-methyl-5alpha-ergosta-8,14,24(28)-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| obtusifoliol + [reduced NADPH-hemoprotein reductase] + O2 | Sorghum bicolor | 4alpha,14alpha-dimethyl-24-methylene-5alpha-cholesta-8-en-3beta-ol | 4alpha-methyl-5alpha-ergosta-8,14,24(28)-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| obtusifoliol + [reduced NADPH-hemoprotein reductase] + O2 | Zea mays | 4alpha,14alpha-dimethyl-5alpha-ergosta-8,24(28)-dien-3beta-ol | 4alpha-methyl-5alpha-ergosta-8,14,24(28)-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| obtusifoliol + [reduced NADPH-hemoprotein reductase] + O2 | Sorghum bicolor | 4alpha,14alpha-dimethyl-5alpha-ergosta-8,24(28)-dien-3beta-ol | 4alpha-methyl-5alpha-ergosta-8,14,24(28)-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | - |
- |
- |
| Candida albicans | - |
- |
- |
| Candida tropicalis | - |
- |
- |
| Homo sapiens | - |
human | - |
| Manihot esculenta | - |
Crantz | - |
| no activity in Escherichia coli | - |
no endogenous sterol 14alpha-demethylase | - |
| Penicillium italicum | - |
filamentous fungi | - |
| Rattus norvegicus | - |
- |
- |
| Saccharomyces cerevisiae | - |
- |
- |
| Schizosaccharomyces pombe | - |
- |
- |
| Sinapis alba | - |
L. | - |
| Sinapis alba L. | - |
L. | - |
| Sorghum bicolor | - |
L., Moench | - |
| Ustilago maydis | - |
- |
- |
| Zea mays | - |
maize | - |
| [Candida] glabrata | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant sorghum CYP51 | Sorghum bicolor |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seedling | - |
Zea mays | - |
| seedling | - |
Sorghum bicolor | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 24,25-dihydrolanosterol + [reduced NADPH-hemoprotein reductase] + O2 | 8-lanosta-3beta-ol | Homo sapiens | 4,4-dimethyl-5alpha-cholesta-8,14-dien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 24,25-dihydrolanosterol + [reduced NADPH-hemoprotein reductase] + O2 | 8-lanosta-3beta-ol | Rattus norvegicus | 4,4-dimethyl-5alpha-cholesta-8,14-dien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 24,25-dihydrolanosterol + [reduced NADPH-hemoprotein reductase] + O2 | 8-lanosta-3beta-ol | Saccharomyces cerevisiae | 4,4-dimethyl-5alpha-cholesta-8,14-dien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 24,25-dihydrolanosterol + [reduced NADPH-hemoprotein reductase] + O2 | 8-lanosta-3beta-ol | Candida albicans | 4,4-dimethyl-5alpha-cholesta-8,14-dien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 24,25-dihydrolanosterol + [reduced NADPH-hemoprotein reductase] + O2 | 8-lanosta-3beta-ol | Candida tropicalis | 4,4-dimethyl-5alpha-cholesta-8,14-dien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 24,25-dihydrolanosterol + [reduced NADPH-hemoprotein reductase] + O2 | 8-lanosta-3beta-ol | [Candida] glabrata | 4,4-dimethyl-5alpha-cholesta-8,14-dien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 24,25-dihydrolanosterol + [reduced NADPH-hemoprotein reductase] + O2 | DHL | Homo sapiens | 4,4-dimethyl-5alpha-cholesta-8,14-dien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 24,25-dihydrolanosterol + [reduced NADPH-hemoprotein reductase] + O2 | DHL | Rattus norvegicus | 4,4-dimethyl-5alpha-cholesta-8,14-dien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 24,25-dihydrolanosterol + [reduced NADPH-hemoprotein reductase] + O2 | DHL | Saccharomyces cerevisiae | 4,4-dimethyl-5alpha-cholesta-8,14-dien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 24,25-dihydrolanosterol + [reduced NADPH-hemoprotein reductase] + O2 | DHL | Candida albicans | 4,4-dimethyl-5alpha-cholesta-8,14-dien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 24,25-dihydrolanosterol + [reduced NADPH-hemoprotein reductase] + O2 | DHL | Candida tropicalis | 4,4-dimethyl-5alpha-cholesta-8,14-dien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 24,25-dihydrolanosterol + [reduced NADPH-hemoprotein reductase] + O2 | DHL | [Candida] glabrata | 4,4-dimethyl-5alpha-cholesta-8,14-dien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 24,25-dihydrolanosterol + [reduced NADPH-hemoprotein reductase] + O2 | 8-lanosten-3beta-ol | Homo sapiens | 4,4-dimethyl-5alpha-cholesta-8,14-dien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 24,25-dihydrolanosterol + [reduced NADPH-hemoprotein reductase] + O2 | 8-lanosten-3beta-ol | Rattus norvegicus | 4,4-dimethyl-5alpha-cholesta-8,14-dien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 24,25-dihydrolanosterol + [reduced NADPH-hemoprotein reductase] + O2 | 8-lanosten-3beta-ol | Saccharomyces cerevisiae | 4,4-dimethyl-5alpha-cholesta-8,14-dien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 24,25-dihydrolanosterol + [reduced NADPH-hemoprotein reductase] + O2 | 8-lanosten-3beta-ol | Candida albicans | 4,4-dimethyl-5alpha-cholesta-8,14-dien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 24,25-dihydrolanosterol + [reduced NADPH-hemoprotein reductase] + O2 | 8-lanosten-3beta-ol | Candida tropicalis | 4,4-dimethyl-5alpha-cholesta-8,14-dien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 24,25-dihydrolanosterol + [reduced NADPH-hemoprotein reductase] + O2 | 8-lanosten-3beta-ol | [Candida] glabrata | 4,4-dimethyl-5alpha-cholesta-8,14-dien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 24,25-dihydrolanosterol + [reduced NADPH-hemoprotein reductase] + O2 | 4,4,14alpha-trimethyl-5alpha-cholesta-8-en-3beta-ol | Homo sapiens | 4,4-dimethyl-5alpha-cholesta-8,14-dien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 24,25-dihydrolanosterol + [reduced NADPH-hemoprotein reductase] + O2 | 4,4,14alpha-trimethyl-5alpha-cholesta-8-en-3beta-ol | Rattus norvegicus | 4,4-dimethyl-5alpha-cholesta-8,14-dien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 24,25-dihydrolanosterol + [reduced NADPH-hemoprotein reductase] + O2 | 4,4,14alpha-trimethyl-5alpha-cholesta-8-en-3beta-ol | Saccharomyces cerevisiae | 4,4-dimethyl-5alpha-cholesta-8,14-dien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 24,25-dihydrolanosterol + [reduced NADPH-hemoprotein reductase] + O2 | 4,4,14alpha-trimethyl-5alpha-cholesta-8-en-3beta-ol | Candida albicans | 4,4-dimethyl-5alpha-cholesta-8,14-dien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 24,25-dihydrolanosterol + [reduced NADPH-hemoprotein reductase] + O2 | 4,4,14alpha-trimethyl-5alpha-cholesta-8-en-3beta-ol | Candida tropicalis | 4,4-dimethyl-5alpha-cholesta-8,14-dien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 24,25-dihydrolanosterol + [reduced NADPH-hemoprotein reductase] + O2 | 4,4,14alpha-trimethyl-5alpha-cholesta-8-en-3beta-ol | [Candida] glabrata | 4,4-dimethyl-5alpha-cholesta-8,14-dien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| eburicol + O2 + [reduced NADPH-hemoprotein reductase] + O2 | - |
Penicillium italicum | 2-(3-hydroxy-4,4,10,13-tetramethyl-2,3,4,5,6,7,10,11,12,13,16,17-dodecahydro-1H-cyclopenta[a]phenanthren-17-yl)-6-methyl-5-methylene-heptanoic acid + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| eburicol + [reduced NADPH-hemoprotein reductase] + O2 | - |
Penicillium italicum | ? + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| lanosterol + [reduced NADPH-hemoprotein reductase] + O2 | lanosta-8,24-dien-3beta-ol | Homo sapiens | 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| lanosterol + [reduced NADPH-hemoprotein reductase] + O2 | lanosta-8,24-dien-3beta-ol | Rattus norvegicus | 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| lanosterol + [reduced NADPH-hemoprotein reductase] + O2 | lanosta-8,24-dien-3beta-ol | Saccharomyces cerevisiae | 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| lanosterol + [reduced NADPH-hemoprotein reductase] + O2 | lanosta-8,24-dien-3beta-ol | Candida albicans | 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| lanosterol + [reduced NADPH-hemoprotein reductase] + O2 | lanosta-8,24-dien-3beta-ol | Candida tropicalis | 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| lanosterol + [reduced NADPH-hemoprotein reductase] + O2 | lanosta-8,24-dien-3beta-ol | [Candida] glabrata | 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| lanosterol + [reduced NADPH-hemoprotein reductase] + O2 | 4,4,14alpha-trimethyl-5alpha-cholesta-8,24-dien-3beta-ol | Homo sapiens | 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| lanosterol + [reduced NADPH-hemoprotein reductase] + O2 | 4,4,14alpha-trimethyl-5alpha-cholesta-8,24-dien-3beta-ol | Rattus norvegicus | 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| lanosterol + [reduced NADPH-hemoprotein reductase] + O2 | 4,4,14alpha-trimethyl-5alpha-cholesta-8,24-dien-3beta-ol | Saccharomyces cerevisiae | 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| lanosterol + [reduced NADPH-hemoprotein reductase] + O2 | 4,4,14alpha-trimethyl-5alpha-cholesta-8,24-dien-3beta-ol | Candida albicans | 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| lanosterol + [reduced NADPH-hemoprotein reductase] + O2 | 4,4,14alpha-trimethyl-5alpha-cholesta-8,24-dien-3beta-ol | Candida tropicalis | 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| lanosterol + [reduced NADPH-hemoprotein reductase] + O2 | 4,4,14alpha-trimethyl-5alpha-cholesta-8,24-dien-3beta-ol | [Candida] glabrata | 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| additional information | substrate specificity | Rattus norvegicus | ? | - |
? | |
| additional information | substrate specificity | Saccharomyces cerevisiae | ? | - |
? | |
| additional information | substrate specificity | Zea mays | ? | - |
? | |
| additional information | substrate specificity | Sorghum bicolor | ? | - |
? | |
| additional information | substrate binding spectra | Sorghum bicolor | ? | - |
? | |
| additional information | biosynthetic enzyme with very narrow substrate specificity | Zea mays | ? | - |
? | |
| additional information | biosynthetic enzyme with very narrow substrate specificity | Sorghum bicolor | ? | - |
? | |
| additional information | enzyme is a multifunctional cytochrome P450, which as the same active site catalyze demethylation in three consecutive NADPH- and O2-dependent hydroxylation reactions, resulting in the elimination of the methyl group as formic acid and the introduction of a double bond at the DELTA14 position | Sorghum bicolor | ? | - |
? | |
| additional information | plant sterol 14alpha-demethylase have high substrate specificity | Zea mays | ? | - |
? | |
| additional information | plant sterol 14alpha-demethylase have high substrate specificity | Sorghum bicolor | ? | - |
? | |
| additional information | catalyzes an essential step in sterol biosynthesis as evidenced by the absence of a 14alpha-methyl group in all known functional sterols, removal of the 14alpha-methyl group is essential | Sorghum bicolor | ? | - |
? | |
| additional information | enzyme of sterol biosynthetic pathway | Homo sapiens | ? | - |
? | |
| additional information | enzyme of sterol biosynthetic pathway | Rattus norvegicus | ? | - |
? | |
| additional information | enzyme of sterol biosynthetic pathway | Saccharomyces cerevisiae | ? | - |
? | |
| additional information | enzyme of sterol biosynthetic pathway | Zea mays | ? | - |
? | |
| additional information | enzyme of sterol biosynthetic pathway | Schizosaccharomyces pombe | ? | - |
? | |
| additional information | enzyme of sterol biosynthetic pathway | Candida albicans | ? | - |
? | |
| additional information | enzyme of sterol biosynthetic pathway | Candida tropicalis | ? | - |
? | |
| additional information | enzyme of sterol biosynthetic pathway | Sorghum bicolor | ? | - |
? | |
| additional information | enzyme of sterol biosynthetic pathway | Ustilago maydis | ? | - |
? | |
| additional information | enzyme of sterol biosynthetic pathway | Penicillium italicum | ? | - |
? | |
| additional information | enzyme of plant sterol, phytosterol, biosynthesis | Zea mays | ? | - |
? | |
| additional information | enzyme of plant sterol, phytosterol, biosynthesis | Sorghum bicolor | ? | - |
? | |
| obtusifoliol + [reduced NADPH-hemoprotein reductase] + O2 | 4alpha,14alpha-dimethyl-24-methylene-5alpha-cholesta-8-en-3beta-ol | Saccharomyces cerevisiae | 4alpha-methyl-5alpha-ergosta-8,14,24(28)-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| obtusifoliol + [reduced NADPH-hemoprotein reductase] + O2 | 4alpha,14alpha-dimethyl-24-methylene-5alpha-cholesta-8-en-3beta-ol | Zea mays | 4alpha-methyl-5alpha-ergosta-8,14,24(28)-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| obtusifoliol + [reduced NADPH-hemoprotein reductase] + O2 | 4alpha,14alpha-dimethyl-24-methylene-5alpha-cholesta-8-en-3beta-ol | Sorghum bicolor | 4alpha-methyl-5alpha-ergosta-8,14,24(28)-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| obtusifoliol + [reduced NADPH-hemoprotein reductase] + O2 | catalyzes 14alpha-demethylation of obtusifoliol | Saccharomyces cerevisiae | 4alpha-methyl-5alpha-ergosta-8,14,24(28)-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| obtusifoliol + [reduced NADPH-hemoprotein reductase] + O2 | catalyzes 14alpha-demethylation of obtusifoliol | Zea mays | 4alpha-methyl-5alpha-ergosta-8,14,24(28)-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| obtusifoliol + [reduced NADPH-hemoprotein reductase] + O2 | catalyzes 14alpha-demethylation of obtusifoliol | Sorghum bicolor | 4alpha-methyl-5alpha-ergosta-8,14,24(28)-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| obtusifoliol + [reduced NADPH-hemoprotein reductase] + O2 | 4alpha,14alpha-dimethyl-5alpha-ergosta-8,24(28)-dien-3beta-ol | Saccharomyces cerevisiae | 4alpha-methyl-5alpha-ergosta-8,14,24(28)-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| obtusifoliol + [reduced NADPH-hemoprotein reductase] + O2 | 4alpha,14alpha-dimethyl-5alpha-ergosta-8,24(28)-dien-3beta-ol | Zea mays | 4alpha-methyl-5alpha-ergosta-8,14,24(28)-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| obtusifoliol + [reduced NADPH-hemoprotein reductase] + O2 | 4alpha,14alpha-dimethyl-5alpha-ergosta-8,24(28)-dien-3beta-ol | Sorghum bicolor | 4alpha-methyl-5alpha-ergosta-8,14,24(28)-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Saccharomyces cerevisiae |
| 30 | - |
assay at | Zea mays |
| 30 | - |
assay at | Sorghum bicolor |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.9 | - |
assay at | Sorghum bicolor |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| cytochrome P450 | - |
Homo sapiens | |
| cytochrome P450 | - |
Rattus norvegicus | |
| cytochrome P450 | - |
Saccharomyces cerevisiae | |
| cytochrome P450 | - |
Zea mays | |
| cytochrome P450 | - |
Arabidopsis thaliana | |
| cytochrome P450 | - |
Schizosaccharomyces pombe | |
| cytochrome P450 | - |
Candida albicans | |
| cytochrome P450 | - |
Candida tropicalis | |
| cytochrome P450 | - |
Sorghum bicolor | |
| cytochrome P450 | - |
Ustilago maydis | |
| cytochrome P450 | - |
Manihot esculenta | |
| cytochrome P450 | - |
Sinapis alba | |
| cytochrome P450 | - |
Penicillium italicum | |
| cytochrome P450 | - |
[Candida] glabrata | |
| heme | hemoprotein | Sorghum bicolor | |
| heme | heme-thiolate enzyme | Homo sapiens | |
| heme | heme-thiolate enzyme | Rattus norvegicus | |
| heme | heme-thiolate enzyme | Saccharomyces cerevisiae | |
| heme | heme-thiolate enzyme | Zea mays | |
| heme | heme-thiolate enzyme | Arabidopsis thaliana | |
| heme | heme-thiolate enzyme | Schizosaccharomyces pombe | |
| heme | heme-thiolate enzyme | Candida albicans | |
| heme | heme-thiolate enzyme | Candida tropicalis | |
| heme | heme-thiolate enzyme | Sorghum bicolor | |
| heme | heme-thiolate enzyme | Ustilago maydis | |
| heme | heme-thiolate enzyme | Manihot esculenta | |
| heme | heme-thiolate enzyme | Sinapis alba | |
| heme | heme-thiolate enzyme | Penicillium italicum | |
| heme | heme-thiolate enzyme | [Candida] glabrata | |
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Release 2023.1 (January 2023)
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